Primary Culture of Human Vestibular SchwannomasNathan M. Schularick 1, J. Jason Clark 1, Marlan R. Hansen 1
1Department of Otolaryngology-Head and Neck, University of Iowa Hospitals and Clinics
Vestibular Schwannomas (VSs) are non-malignant tumors of Schwann cell (SC) origin, associated with mutations in the NF2 tumor suppressor gene. We report a reproducible, efficient protocol for primary human VS cell culture that allows for molecular and cellular experimental manipulation and analysis and recapitulates the heterogeneous nature of human disease.
