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Jiangsu Academy of Agricultural Sciences

6 ARTICLES PUBLISHED IN JoVE

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Biology

Measuring Spatial and Temporal Ca2+ Signals in Arabidopsis Plants
Xiaohong Zhu 1, Aaron Taylor 2, Shenyu Zhang 1, Dayong Zhang 1,3, Ying Feng 1,4, Gaimei Liang 1,5, Jian-Kang Zhu 1,6
1Department of Horticulture and Landscape Architecture, Purdue University, 2Bindley Bioscience Center, Purdue University, 3Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences, 4College of Environmental & Resource Science, Zhejiang University, 5Dryland Agriculture Research Centre, Shanxi Academy of Agricultural Sciences, 6Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences

Ca2+ signaling regulates diverse biological processes in plants. Here we present approaches for monitoring abiotic stress induced spatial and temporal Ca2+ signals in Arabidopsis cells and tissues using the genetically encoded Ca2+ indicators Aequorin or Case12.

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Environment

Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples
Huan Chen *1,2, Ian Arthur Palmer *1, Jian Chen 1,2, Ming Chang 1,2, Stephen L. Thompson 3, Fengquan Liu 2, Zheng Qing Fu 1
1Department of Biological Sciences, University of South Carolina, 2Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, 3Department of Instructions and Teacher Education, University of South Carolina

Citrus Greening is a particularly destructive disease affecting citrus crops globally. Presented here is a simple method using PCR and genomic DNA extraction of citrus leaf tissue for the accurate and precise identification of the citrus greening pathogen, Candidatus liberibacter spp.

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Cancer Research

Patient-derived Heterogeneous Xenograft Model of Pancreatic Cancer Using Zebrafish Larvae as Hosts for Comparative Drug Assessment
Lei Wang *1, Huan Chen *2,3, Fei Fei *1, Xianfeng He 2,3, Shaoyang Sun 1, Kunpeng Lv 1, Bo Yu 2,3, Jiang Long 2,4,5,6, Xu Wang 1
1Department of Biochemistry and Molecular Biology, Key Laboratory of Metabolism and Molecular Medicine, Ministry of Education, School of Basic Medical Sciences, Fudan University, 2National Human Genetic Resources Sharing Service Platform (2005DKA21300), 3Fudan University Shanghai Cancer Center, 4Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, 5Department of Oncology, Shanghai Medical College, Fudan University, 6Pancreatic Cancer Institute, Fudan University

This protocol describes optimization procedures in a virus-based dual fluorescence-labeled tumor xenograft model using larval zebrafish as hosts. This heterogeneous xenograft model mimics the tissue composition of pancreatic cancer microenvironment in vivo and serves as a more precise tool for assessing drug responses in personalized zPDX (zebrafish patient-derived xenograft) models.

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Genetics

CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation
Linyuan Ma *1, Lydia Jang *1, Jian Chen 2, Jun Song 1, Dongshan Yang 1, Jifeng Zhang 1, Y. Eugene Chen 1, Jie Xu 1
1Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical Center, 2Celetrix LLC

Presented here is a protocol for efficient CRISPR/Cas9 ribonucleoprotein-mediated gene editing in mammalian cells using tube electroporation.

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Medicine

A Training and Testing System for Performing Vascular Reconstruction In Vitro
Yue Wang 1,2, Lijun Mu 3, Wei Zhang 1,2, Huan Chen 1,2, Qingshan Li 1,2, Aihua Shi 1, Bo Tang 2, Xiaogang Zhang 1,2, Dinghui Dong 1,2, Yi Lv 1,2
1National Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine, First Affiliated Hospital of Xi'an Jiaotong University, 2Department of Hepatobiliary Surgery, First Affiliated Hospital of Xi'an Jiaotong University, 3Department of General Surgery, First Affiliated Hospital of Xi'an Jiaotong University

Here we present a training and testing system where a trainee can complete manual vascular reconstruction in vitro individually using a magnetic anchoring technique. The system can also be used to test the quality of reconstruction.

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Immunology and Infection

Use of Fimbrial Rod for F18ab Fimbriae+ STEC Colonization to Host Cells
Mingxu Zhou *1,2, Qiangde Duan *1,3, Yang Yang 1,3, Guoqiang Zhu 1,3
1College of Veterinary Medicine, Yangzhou University, 2Institute of Veterinary Immunology & Engineering, Jiangsu Academy of Agricultural Sciences, 3Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses

Here we present a protocol to study the function of fimbriae in bacterial colonization.

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