Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentationJun Imai 1, Mayu Otani 1, Takahiro Sakai 1, Shinichi Hatta 1
1Laboratory of Physiological Chemistry, Faculty of Pharmacy, Takasaki University of Health and Welfare
The method described here is a new vesicle isolation protocol, which allows for the purification of the cellular compartments where exogenous antigens are processed by endoplasmic reticulum-associated degradation in cross-presentation.