A Guide to Production, Crystallization, and Structure Determination of Human IKK1/αSmarajit Polley 1,2, De-Bin Huang 1, Tapan Biswas 1, Gourisankar Ghosh 1
1Department of Chemistry & Biochemistry, University of California, San Diego, 2Department of Biophysics, Bose Institute
IκB Kinase 1/α (IKK1/α CHUK) is a Ser/Thr protein kinase that is involved in a myriad of cellular activities primarily through activation of NF-κB transcription factors. Here, we describe the main steps necessary for the production and crystal structure determination of this protein.