The University of Tokyo Hospital

3 ARTICLES PUBLISHED IN JoVE

Medicine

Three-dimensional Co-culture Model for Tumor-stromal Interaction

Masafumi Horie^1,2, Akira Saito^1,3, Yoko Yamaguchi⁴, Mitsuhiro Ohshima⁵, Takahide Nagase¹

¹Department of Respiratory Medicine, Graduate School of Medicine, The University of Tokyo, ²Department of Clinical Laboratory, Graduate School of Medicine, The University of Tokyo, ³Division for Health Service Promotion, The University of Tokyo, ⁴Department of Biochemistry, Nihon University School of Dentistry, ⁵Department of Biochemistry, Ohu University School of Pharmaceutical Sciences

Here we present a protocol to co-culture in three-dimensions, which is useful for investigating multicellular interactions and extracellular matrix-dependent modulation of cancer cell behavior. In this experimental model, cancer cells are cultured on collagen gels embedded with human cancer-associated fibroblasts.

Developmental Biology

Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition

Yu Mikami^1,2, Hirotaka Matsuzaki², Hideyuki Takeshima², Kosuke Makita², Yasuhiro Yamauchi², Takahide Nagase²

¹Department of Clinical Laboratory, The University of Tokyo Hospital, ²Department of Respiratory Medicine, The University of Tokyo Hospital

Here, we describe the development and application of a gel contraction assay for evaluating contractile function in mesenchymal cells that underwent epithelial-mesenchymal transition.

Biology

A Pacing-Controlled Procedure for the Assessment of Heart Rate-Dependent Diastolic Functions in Murine Heart Failure Models

Genri Numata^1,2, Eiki Takimoto^1,3

¹Department of Cardiovascular Medicine, The University of Tokyo Hospital, ²Department of Therapeutic Strategy for Heart Failure, Graduate School of Medicine, The University of Tokyo, ³Division of Cardiology, Department of Medicine, The Johns Hopkins Medical Institutions

The present protocol describes obtaining the pressure-volume relationship through transesophageal pacing, which serves as a valuable tool in evaluating diastolic function in mouse models of heart failure.