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Yunnan University

7 ARTICLES PUBLISHED IN JoVE

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Medicine

Catheterization of the Carotid Artery and Jugular Vein to Perform Hemodynamic Measures, Infusions and Blood Sampling in a Conscious Rat Model
Jing Feng 1, Yvonne Fitz 1, Yan Li 1, Melinda Fernandez 1, Irene Cortes Puch 1, Dong Wang 1, Stephanie Pazniokas 2, Brandon Bucher 3, Xizhong Cui 1, Steven B. Solomon 1
1Critical Care Medicine Department, Clinical Center, National Institutes of Health, 2Harvard Apparatus, 3ADInstruments

Vascular accesses to measure hemodynamics, provide fluids and perform blood sampling are important to any small animal model study. We present a technique for implanting catheters into the carotid artery and the common jugular vein in an anesthetized rat for connecting to a system to perform monitoring, infusions and sampling.

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Engineering

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
Holly D. Carlton 1, John W. Elmer 1, Yan Li 2, Mario Pacheco 2, Deepak Goyal 2, Dilworth Y. Parkinson 3, Alastair A. MacDowell 3
1Materials Engineering Division, Lawrence Livermore National Laboratory, 2Assembly Test and Technology Development Failure Analysis Labs, Intel Corporation, 3Advanced Light Source, Lawrence Berkeley National Laboratory

For this study synchrotron radiation micro-tomography, a non-destructive three-dimensional imaging technique, is employed to investigate an entire microelectronic package with a cross-sectional area of 16 x 16 mm. Due to the synchrotron's high flux and brightness the sample was imaged in just 3 min with an 8.7 µm spatial resolution.

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Bioengineering

Probing the Roles of Physical Forces in Early Chick Embryonic Morphogenesis
Yan Li *1, Hannah Grover *1, Eric Dai 2, Kevin Yang 1, Zi Chen 1
1Thayer School of Engineering, Dartmouth College, 2Department of Bioengineering, University of Pennsylvania

Here, we present a protocol introducing a set of new ex-ovo experiments and physical modeling approaches for studying the mechanics of morphogenesis during early chick embryonic brain torsion.

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Neuroscience

Purification of Fibroblasts and Schwann Cells from Sensory and Motor Nerves in Vitro
Qianru He 1, Fanhui Yu 1, Yan Li 1, Junjie Sun 1, Fei Ding 1
1Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-Innovation Center of Neuroregeneration, Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair, Nantong University

Here, we present a method to purify fibroblasts and Schwann cells from sensory and motor nerves in vitro.

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Biochemistry

Transplantation of Neonatal Mouse Cardiac Macrophages into Adult Mice
Yandong Li 1, Jie Feng 1, Yan Li 1, Jianqiu Pei 1, Shengshou Hu 1, Yu Nie 1,2
1Fuwai Hospital & Chinese Academy of Medical Sciences, State Key Laboratory of Cardiovascular Disease, Cardiovascular Institute, National Center for Cardiovascular Diseases, Peking Union Medical College, 2National Health Commission Key Laboratory of Cardiovascular Regenerative Medicine, Fuwai Central-China Hospital, Central-China Subcenter of National Center for Cardiovascular Diseases

We provide a protocol for neonatal cardiac macrophage separation and transplantation into an adult mouse heart, which could be a promising way to promote cardiac repair.

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Neuroscience

Organotypic Retinal Explant Cultures from Macaque Monkey
Wenrong Xu 1, Yujie Dong 1, Yan Li 1, Zhulin Hu 1, François Paquet-Durand 2, Kangwei Jiao 1
1Yunnan Eye Institute & Key Laboratory of Yunnan Province, Yunnan Eye Disease Clinical Medical Center, Affiliated Hospital of Yunnan University, Yunnan University, 2Institute for Ophthalmic Research, Eberhard-Karls-Universität Tübingen

Retinal explants obtained from wild-type macaques were cultured in vitro. Retinal degeneration and the cGMP-PKG signaling pathway was induced using the PDE6 inhibitor zaprinast. cGMP accumulation in the explants at different zaprinast concentrations was verified using immunofluorescence.

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Cancer Research

Multiplex Cyclic Fluorescent Immunohistochemistry
Yun Chen *1, Hui Zhang *2, Yaodong Fan 3, Liu Yang 4,5, Yan Dong *1
1Department of Pathology, The Third Affiliated Hospital of Kunming Medical University & Yunnan Cancer Hospital, 2Department of Gastroenterology, Wu Han NO.1 Hospital, 3Department of Neurosurgery, The Third Affiliated Hospital of Kunming Medical University & Yunnan Cancer Hospital, 4Departments of Biomedical Research Center, The Affiliated Calmette Hospital of Kunming Medical University & The First Hospital of Kunming, 5School of Medical, Yunnan University

Multiplex cyclic immunohistochemistry allows in situ detection of multiple markers simultaneously using repeated antigen-antibody incubation, image scanning, and image alignment and integration. Here, we present the operating protocol for identifying immune cell substrates with this technology in lung cancer and paired brain metastasis samples.

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