Rush University Medical Center

5 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays

Zachary B. Davis¹, Jeffrey P. Ward¹, Edward Barker¹

¹Department of Immunology and Microbiology, Rush University Medical Center

Cytotoxicity assays to measure natural killer cell lytic responses to HIV-infected cells is limited by the purity of the target cells. We demonstrate here the isolation of a highly purified population of HIV-1 infected primary T-cell blasts by taking advantage of HIV-1 s ability to down-modulate CD4.

Biology

Assessment of Calcium Sparks in Intact Skeletal Muscle Fibers

Ki Ho Park¹, Noah Weisleder², Jingsong Zhou³, Kristyn Gumpper¹, Xinyu Zhou¹, Pu Duann⁴, Jianjie Ma¹, Pei-Hui Lin¹

¹Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, ²Department of Physiology and Cell Biology, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, ³Department of Molecular Biophysics and Physiology, Rush University Medical Center, ⁴Department of Internal Medicine, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center

Described here is a method to directly measure calcium sparks, the elementary units of Ca²⁺ release from sarcoplasmic reticulum in intact skeletal muscle fibers. This method utilizes osmotic-stress-mediated triggering of Ca²⁺ release from ryanodine receptor in isolated muscle fibers. The dynamics and homeostatic capacity of intracellular Ca²⁺ signaling can be employed to assess muscle function in health and disease.

Medicine

Rat Model of the Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy (ALPPS) Procedure

Erik Schadde^1,2,3, Martin Hertl², Stefan Breitenstein³, Beatrice Beck-Schimmer^1,4, Martin Schläpfer^1,4

¹Institute of Physiology - Center for Integrative Human Physiology, University of Zurich, ²Department of Surgery, Rush University Medical Center, ³Department of Surgery, Cantonal Hospital Winterthur, ⁴Institute of Anesthesiology, University and University Hospital Zurich

Inducing rapid liver hypertrophy using Associating Liver Partition and Portal vein ligation for a Staged hepatectomy (ALPPS) has been proposed for resection of borderline resectable liver tumors. This model may elucidate mechanisms involved in rapid hypertrophy and allows testing of drugs that promote or block the acceleration of regeneration.

Medicine

Estimation of Nephron Number in Whole Kidney using the Acid Maceration Method

Sarah M. Peterson¹, Xuexiang Wang², Ashley C. Johnson¹, Ian D. Coate¹, Michael R. Garrett^1,3, Sean P. Didion^1,4

¹Department of Pharmacology and Toxicology, The University of Mississippi Medical Center, ²Department of Medicine, Division of Nephrology, Rush University Medical Center, ³Department of Medicine, Division of Nephrology, The University of Mississippi Medical Center, ⁴Department of Neurology, The University of Mississippi Medical Center

Estimates of whole kidney nephron number are important clinically and experimentally, as there is an inverse association between nephron number and an enhanced risk of renal and cardiovascular disease.Herein, the use of the acid maceration method, which provides fast and reliable estimates of whole kidney nephron number, is demonstrated.

Medicine

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

Cristina L. Fhied^*1, Imad Tarhoni^*1, David Gerard¹, Grant M. Lewin¹, Hita Moudgalya¹, Jeffrey R. Schneider², Jeffrey A. Borgia^1,3

¹Department of Anatomy & Cell Biology, Rush University Medical Center, ²Department of Microbial Pathogens and Immunity, Rush University Medical Center, ³Department of Pathology, Rush University Medical Center

This article describes a convenient method for monitoring dynamic changes in antibody titers for two immunoglobulin isotypes concurrently (either IgA, IgM, or IgG) resulting from an immune response to SARS-CoV-2 infection or vaccination. This 'Multianalyte Covid-19 Immune Response Panel' employs three indirect immunoassays built on coded microspheres that are read using a flow-based multiplex reader with 'dual-channel' capability.