JoVE Logo

Sign In

Nantong University

13 ARTICLES PUBLISHED IN JoVE

image

Medicine

Catheterization of the Carotid Artery and Jugular Vein to Perform Hemodynamic Measures, Infusions and Blood Sampling in a Conscious Rat Model
Jing Feng 1, Yvonne Fitz 1, Yan Li 1, Melinda Fernandez 1, Irene Cortes Puch 1, Dong Wang 1, Stephanie Pazniokas 2, Brandon Bucher 3, Xizhong Cui 1, Steven B. Solomon 1
1Critical Care Medicine Department, Clinical Center, National Institutes of Health, 2Harvard Apparatus, 3ADInstruments

Vascular accesses to measure hemodynamics, provide fluids and perform blood sampling are important to any small animal model study. We present a technique for implanting catheters into the carotid artery and the common jugular vein in an anesthetized rat for connecting to a system to perform monitoring, infusions and sampling.

image

Engineering

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
Holly D. Carlton 1, John W. Elmer 1, Yan Li 2, Mario Pacheco 2, Deepak Goyal 2, Dilworth Y. Parkinson 3, Alastair A. MacDowell 3
1Materials Engineering Division, Lawrence Livermore National Laboratory, 2Assembly Test and Technology Development Failure Analysis Labs, Intel Corporation, 3Advanced Light Source, Lawrence Berkeley National Laboratory

For this study synchrotron radiation micro-tomography, a non-destructive three-dimensional imaging technique, is employed to investigate an entire microelectronic package with a cross-sectional area of 16 x 16 mm. Due to the synchrotron's high flux and brightness the sample was imaged in just 3 min with an 8.7 µm spatial resolution.

image

Genetics

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
Desiree-Faye Kaixin Toh *1, Kiran M. Patil *1, Gang Chen 1
1Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University

We report the protocols for the synthesis and purification of Peptide Nucleic Acid (PNA) oligomers incorporating modified residues. The biochemical and biophysical methods for the characterization of the recognition of RNA duplexes by the modified PNAs are described.

image

Medicine

Surgical Treatment for Benign Prostatic Hyperplasia: Holmium Laser Enucleation of the Prostate (HoLEP).
Huan Xu *1, Xiang Wan *1, Meng Gu 1, Yanbo Chen 1, Qiling Shi 1, Qi Chen 1, Zhong Wang 1
1Department of Urology, Shanghai 9th People's Hospital, Shanghai Jiaotong University School of Medicine

Here we present a safe and efficient protocol, holmium laser enucleation of the prostate, to treat benign prostatic hyperplasia.

image

Bioengineering

Probing the Roles of Physical Forces in Early Chick Embryonic Morphogenesis
Yan Li *1, Hannah Grover *1, Eric Dai 2, Kevin Yang 1, Zi Chen 1
1Thayer School of Engineering, Dartmouth College, 2Department of Bioengineering, University of Pennsylvania

Here, we present a protocol introducing a set of new ex-ovo experiments and physical modeling approaches for studying the mechanics of morphogenesis during early chick embryonic brain torsion.

image

Neuroscience

An Invasive Method for the Activation of the Mouse Dentate Gyrus by High-frequency Stimulation
Zhe Zhao 1, Haitao Wu 1,2
1Department of Neurobiology, Beijing Institute of Basic Medical Sciences, 2Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University

This protocol shows how to set up a reliable HFS method in mice. Neurons throughout the hippocampal dentate gyrus are electrically stimulated by HFS directly and indirectly in vivo. Neuronal activity and molecular signaling are examined by c-fos and Notch1 immunofluorescent staining, respectively; neurogenesis is quantified by bromodeoxyuridine labeling assay.

image

Neuroscience

Purification of Fibroblasts and Schwann Cells from Sensory and Motor Nerves in Vitro
Qianru He 1, Fanhui Yu 1, Yan Li 1, Junjie Sun 1, Fei Ding 1
1Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-Innovation Center of Neuroregeneration, Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair, Nantong University

Here, we present a method to purify fibroblasts and Schwann cells from sensory and motor nerves in vitro.

image

Medicine

Using Q Suture to Enhance Resistance to Gap Formation and Tensile Strength of Repaired Flexor Tendons
Wei Feng Mao 1, Ya Fang Wu 2
1Department of Anatomy, Medical School, Nantong University, 2Department of Hand surgery, Affiliated Hospital of Nantong University

Here, we present a “Q” suture technique that can be performed in tendon repair and its effects on the gap formation and tensile strength of the repaired tendons. Q suture is shown to be efficient in enhancing the tensile resistance and tendon repair strength.

image

Biochemistry

Transplantation of Neonatal Mouse Cardiac Macrophages into Adult Mice
Yandong Li 1, Jie Feng 1, Yan Li 1, Jianqiu Pei 1, Shengshou Hu 1, Yu Nie 1,2
1Fuwai Hospital & Chinese Academy of Medical Sciences, State Key Laboratory of Cardiovascular Disease, Cardiovascular Institute, National Center for Cardiovascular Diseases, Peking Union Medical College, 2National Health Commission Key Laboratory of Cardiovascular Regenerative Medicine, Fuwai Central-China Hospital, Central-China Subcenter of National Center for Cardiovascular Diseases

We provide a protocol for neonatal cardiac macrophage separation and transplantation into an adult mouse heart, which could be a promising way to promote cardiac repair.

image

JoVE Journal

Wide-Field, Real-Time Imaging of Local and Systemic Wound Signals in Arabidopsis
Takuya Uemura 1, Jiaqi Wang 1, Yuri Aratani 1, Simon Gilroy 2, Masatsugu Toyota 1,2
1Department of Biochemistry and Molecular Biology, Saitama University, 2Department of Botany, University of Wisconsin

Extracellular glutamate-triggered systemic calcium signaling is critical for the induction of plant defense responses to mechanical wounding and herbivore attack in plants. This article describes a method to visualize the spatial and temporal dynamics of both these factors using Arabidopsis thaliana plants expressing calcium- and glutamate-sensitive fluorescent biosensors.

image

Neuroscience

Primary Microglia Isolation from Postnatal Mouse Brains
Siling Du *1,2, Shanshan Xiong *3, Xiangjuan Du 4, Ti-Fei Yuan 1, Bo Peng 3,4,5, Yanxia Rao 1
1Shanghai Key Laboratory of Psychotic Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, 2Division of Biology and Biomedical Science, Washington University in St. Louis, 3Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, 4Department of Neurosurgery, Huashan Hospital, Institute for Translational Brain Research, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Fudan University, 5Co-innovation Center of Neuroregeneration, Nantong University

Primary cell culture is one of the primarily used approaches for studying microglial biology in vitro. Here, we developed a method for simple and rapid microglia isolation from the mouse postnatal day 1 (P1) to P4.

image

Neuroscience

In Situ Hybridization Combined with Immunohistochemistry in Cryosectioned Zebrafish Embryos
Jiaqi Wang *1,2, Rui Chai *3, Xiaoxia Fang 2, Jiajia Gu 4, Wanqing Xu 2, Qi Chen 1, Gang Chen 2,3, Shunxing Zhu 5, Yan Jin 1
1School of Life Sciences, Nantong University, 2Medical School, Nantong University, 3Key Laboratory of Neuroregeneration of Jiangsu Province and Ministry of Education, Co-Innovation Center of Neuroregeneration, Nantong University, 4Nantong Maternal and Child Health Care Hospital, 5Laboratory Animals Center, Nantong University

This protocol describes how to obtain images by combining in situ hybridization and immunohistochemistry of zebrafish embryonic sections. In situ hybridization was performed prior to cryosectioning, followed by antibody staining. It is useful to detect the expression patterns of two genes in zebrafish if there is a paucity of antibodies.

image

Engineering

Swin-PSAxialNet: An Efficient Multi-Organ Segmentation Technique
Lei Ma 1, Dian Zhang 1, Zhaoxin Wang 1, Jincong Han 1, Xing Wang 1, Xin Zhou 2, Wenwen Yang 1, Peihua Lu 2
1School of Information Science and Technology, Nantong University, 2The Oncology and Hematology Department of Wuxi People's Hospital of Nanjing Medical University, Wuxi Medical Center, Nanjing Medical University

The present protocol describes an efficient multi-organ segmentation method called Swin-PSAxialNet, which has achieved excellent accuracy compared to previous segmentation methods. The key steps of this procedure include dataset collection, environment configuration, data preprocessing, model training and comparison, and ablation experiments.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved