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Kwangwoon University

4 ARTICLES PUBLISHED IN JoVE

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Biology

A Novel Nicotinamide Adenine Dinucleotide Correction Method for Intracellular Ca2+ Measurement with Fura-2-Analog in Live Cells
Jeong Hoon Lee 1, Jeong Mi Ha 1, Quynh Mai Ho 1, Chae Hun Leem 1,2,3
1Department of Physiology, University of Ulsan College of Medicine/Asan Medical Center, 2Asan Medical Center, 3Asan Medical Institute of Convergence Science and Technology

Due to the spectral overlapping of the excitation and emission wavelengths of NADH and fura-2 analogs, the signal interference from both chemicals in live cells is unavoidable during quantitative measurement of [Ca2+]. Thus, a novel online correction method of NADH signal interference to measure [Ca2+] was developed.

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Engineering

Recording Ultra-Realistic Full-Color Analog Holograms for Use in a Moving Hologram Display
Philippe Gentet 1, Yves Gentet 2, Lyoung-Hui Kim 1, Kwang-Jib Kim 1, Seung-Hyun Lee 3
1Department of Plasma Bio Display, Kwangwoon University, 2Bordeaux Holography, 3Department of Ingenium College, Kwangwoon University

We present a protocol to record a set of ultra-realistic full-color analog holograms, showing the same brightness, transparency, and homogeneous colors, on ultra-fine-grain silver-halide holographic emulsions for the fabrication of a dynamic holographic 3D display.

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Biochemistry

Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes
Dohwan Lee *1, Kyung Wook Wee *1, Hyerin Kim *1, Sung Il Han 1, Seungmin Kwak 1,2, Dae Sung Yoon 3, Jeong Hoon Lee 1
1Department of Electrical Engineering, Kwangwoon University, 2Korea Institute of Science and Technology (KIST), 3School of Biomedical Engineering, Korea University

Presented is a protocol to fabricate a paper-based device for the effective enrichment and isolation of microvesicles and exosomes.

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Bioengineering

High-Performance Graphene-Modified Sensing Chip for SARS-CoV-2 Detection
Parshant Kumar Sharma 1,2,3, Ebrahim Mostafavi 4,5, Nam-Young Kim 1,2,3, Thomas J. Webster 6, Ajeet Kaushik 7,8
1RFIC Bio Centre, Kwangwoon University, 2Department of Electronics Engineering, Kwangwoon University, 3NDAC Centre, Kwangwoon University, 4Stanford Cardiovascular Institute, Stanford University School of Medicine, 5Department of Medicine, Stanford University School of Medicine, 6Department of Biomedical Engineering, Hebei University of Technology, 7NanoBioTech Laboratory, Department of Environmental Engineering, Florida Polytechnic University, 8School of Engineering, University of Petroleum and Energy Studies (UPES)

The present protocol describes the fabrication of low-cost biosensing prototypes based on useful nanosystems for accurately detecting viral proteins (at the Fg level). Such a tiny sensor platform allows for point-of-care applications that can be integrated with the Internet of Medical Things (IoMT) to meet telemedicine objectives.

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