Southern California Coastal Water Research Project Authority

1 ARTICLES PUBLISHED IN JoVE

Environment

Screening for Endocrine Activity in Water Using Commercially-available In Vitro Transactivation Bioassays

Alvine C. Mehinto¹, B. Sumith Jayasinghe², Darcy R. Vandervort¹, Nancy D. Denslow², Keith A. Maruya¹

¹Southern California Coastal Water Research Project Authority, ²Department of Physiological Sciences, University of Florida

A protocol to screen for endocrine activity in organic extracts of water samples, including treated wastewater effluent and surface (receiving) water, was adapted using commercially available division-arrested ("freeze and thaw") in vitro transactivation bioassays.