Qingdao Agricultural University

2 ARTICLES PUBLISHED IN JoVE

Biology

Staining the Cytoplasmic Ca²⁺ with Fluo-4/AM in Apple Pulp

Lina Qiu¹, Daqing Huang¹, Yongzhang Wang¹, Haiyong Qu¹

¹College of Horticulture, Qingdao Agricultural University

Isolated protoplasts of apple pulp cells were loaded with a calcium fluorescent reagent to detect cytoplasmic Ca²⁺ concentration.

Immunology and Infection

Draining Lymph Node Metastasis Model for Assessing the Dynamics of Antigen-Specific CD8⁺ T Cells During Tumorigenesis

Yan Zhang^*1, Xingxing Su^*2, Lisha Wang², Zhengliang Yue², Qiao Liu³, Ling Ran², Shun Lei², Jianjun Hu², Lifan Xu², Lilin Ye², Ping Ji⁴, Guimei Li¹, Qizhao Huang⁵, Shuqiong Wen⁴

¹College of Veterinary Medicine, Qingdao Agricultural University, ²Institute of Immunology, Third Military Medical University, ³Institute of Cancer, Xinqiao Hospital, Third Military Medical University, ⁴Stomatological Hospital of Chongqing Medical University, ⁵Institute of life science, Chongqing Medical University

The experimental design presented here provides a useful reproductive model for the studies of antigen-specific CD8⁺ T cells during lymph node (LN) metastasis, which excludes the perturbation of bystander CD8⁺ T cells.