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Wuhan University of Science and Technology

3 ARTICLES PUBLISHED IN JoVE

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Engineering

Nerve Stem Cell Differentiation by a One-step Cold Atmospheric Plasma Treatment In Vitro
Zilan Xiong *1, Shasha Zhao *2, Xu Yan 3
1State Key Laboratory of Advanced Electromagnetic Engineering and Technology, Huazhong University of Science and Technology, 2College of Life Science and Health, Wuhan University of Science and Technology, 3Department of Pathophysiology, Beijing Neurosurgical Institute/Beijing Tiantan Hospital, Capital Medical University

This protocol aims to provide detailed experimental steps of a cold atmospheric plasma treatment on neural stem cells and immunofluorescence detection for differentiation enhancement.

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Neuroscience

Neuronal Differentiation from Mouse Embryonic Stem Cells In vitro
Xiang Mao *1, Shasha Zhao *2
1Wuhan Center for Disease Control and Prevention, 2College of Life Science and Health, Wuhan University of Science and Technology

Here, we established a low cost and easy to operate method that directs fast and efficient differentiation from embryonic stem cells into neurons. This method is suitable for popularization among laboratories and can be a useful tool for neurological research.

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Medicine

Transmission Electron Microscopy: A Surgical Pathology Tool for Neuroblastoma
Consolato M. Sergi *1,2,3,4, Janice Patry *1, Harry Coenraad 1, Jeff McClintock 1, Rod Nicholls 1, Hans-Jörg Steiner 5, Gregor Mikuz 5
1Anatomic Pathology Division of EORLA, Children's Hospital of Eastern Ontario, University of Ottawa, 2Department of Laboratory Medicine and Pathology, University of Alberta, 3Department of Pediatrics, University of Alberta, Stollery Children's Hospital, 4Department of Orthopedics, Tianyou Hospital, Wuhan University of Science and Technology, 5Institute of Pathology, Medical University of Innsbruck

Pediatric small round blue cell tumors are an intriguing and challenging collection of neoplasms. Therefore, transmission electron microscopy (TEM) and professional knowledge of pediatric tumors can be extremely valuable in surgical pathology. Here, we present a protocol to perform TEM for diagnosing neuroblastoma, one of the most common solid tumors in childhood.

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