Imaging Cell Viability on Non-transparent Scaffolds — Using the Example of a Novel Knitted Titanium ImplantGauri Tendulkar 1, Phillip Grau 1, Patrick Ziegler 1,2, Alfred Buck, Sr. 3, Alfred Buck, Jr. 3, Andreas Badke 1,2, Hans-Peter Kaps 1,2, Sabrina Ehnert 1, Andreas K. Nussler 1
1Siegfried Weller Institute for Trauma Research at the BG Trauma Center, Eberhard Karls Universität Tübingen, 2Department of Orthopaedics, BG Trauma-Center, 3Buck GmbH and Co.KG
Here we present a fluorophore based imaging technique to detect cell viability on a non-transparent titanium scaffold as well as to detect glimpses of the scaffold impurities. This protocol troubleshoots the drawback of imaging cell-cell or cell-metal interactions on non-transparent scaffolds.
