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The second affiliated hospital of Guangzhou university of Chinese Medicine

11 ARTICLES PUBLISHED IN JoVE

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Behavior

Bouncing Ball with a Uniformly Varying Velocity in a Metronome Synchronization Task
Yingyu Huang *1, Li Gu *1, Junkai Yang *1, Xiang Wu 1
1Department of Psychology, Sun Yat-Sen University

The purpose of this protocol is to introduce the application of a bouncing ball with a uniformly varying velocity in a metronome synchronization task.

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Medicine

A Detailed Protocol for Physiological Parameters Acquisition and Analysis in Neurosurgical Critical Patients
Xiang Wu 1,2, Guoyi Gao 1,2, Junfeng Feng 1,2, Qing Mao 1,2, Jiyao Jiang 1,2
1Department of Neurosurgery, Renji Hospital, Shanghai Jiaotong University School of Medicine, 2Shanghai Institute of Head Trauma

Recently, we have developed a portable multimodality monitoring system for the monitoring of various physiological parameters in neurosurgical critical patients. Detailed protocols on how to use this multimodality monitoring system are presented here.

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Medicine

A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation
Guihao Chen 1, Yuejin Yang 1, Chuansheng Xu 2, Shuo Gao 2
1State Key Laboratory of Cardiovascular Disease, Department of Cardiology, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, 2State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College

Here, we present a protocol that uses JC-1 dye to assess the mitochondrial membrane potential of cells after being exposed to hypoxia/reoxygenation with or without a protective agent.

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JoVE Journal

A 100 KW Class Applied-field Magnetoplasmadynamic Thruster
Baojun Wang 1, Haibin Tang 2, Yibai Wang 1, Chao Lu 1, Cheng Zhou 3, Yangyang Dong 1, Ge Wang 3, Yuntian Cong 3, Daniel Luu 4, Jinbin Cao 2
1Key Laboratory of Spacecraft Design Optimization & Dynamic Simulation Technologies of Ministry of Education, School of Astronautics, Beihang University, 2Key Laboratory of Spacecraft Design Optimization & Dynamic Simulation Technologies of Ministry of Education, School of Space and Environment, Beihang University, 3Beijng Institute of Control Engineering, 4School of Aerospace Engineering and Geodesy, University of Stuttgart

The goal of this protocol is to introduce the design of a 100 kW class applied-field magnetoplasmadynamic thruster and relevant experimental methods.

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Neuroscience

In Vivo Methods to Assess Retinal Ganglion Cell and Optic Nerve Function and Structure in Large Animals
Qian Ye *1, Zhonghao Yu *1, Tian Xia *1, Shengjian Lu 1, Jiaying Sun 1, Mengyun Li 1, Yu Xia 1, Si Zhang 1, Wencan Wu 1, Yikui Zhang 1
1The Eye Hospital, School of Ophthalmology & Optometry, Wenzhou Medical University

Here we demostrate several in vivo tests (flash visual evoked potential, pattern electroretinogram and optic coherence tomography) in goat and rhesus macaque to understand the structure and function of the optic nerve and its neurons.

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Medicine

Laparoscopic Radical Antegrade Modular Pancreatosplenectomy via Dorsal-Caudal Artery Approach for Pancreatic Neck-Body Cancer
Zhantao Shen 1,2, Guihao Chen 1,2, Chunbao Zhu 2, Xiang Wu 1,2, Yifeng Liu 1,2, Zhangyuanzhu Liu 1,2, Zhijian Tan 1,2, Xiaosheng Zhong 1,2
1The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 2The Second Clinical College of Guangzhou University of Chinese Medicine

With advancements in laparoscopic techniques, laparoscopic radical antegrade modular pancreatosplenectomy (L-RAMPS) has been widely recognized. However, owing to several technical difficulties in this procedure, the artery-first approach in L-RAMPS still remains uncommon. Here, we developed the dorsal-caudal artery approach for L-RAMPS, which might be safe and beneficial for pancreatic neck tumors.

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Medicine

Laparoscopic Pancreatoduodenectomy for Pancreatic Cancer Using In-Situ No-Touch Isolation Technique
Zhantao Shen 1,2, Xiang Wu 1,2, Guihao Chen 1,2, Yifeng Liu 1,2, Chunbao Zhu 2, Fanxing Huang 2, Zhijian Tan 1,2, Xiaosheng Zhong 1,2
1The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 2The Second Clinical College of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou University of Chinese Medicine

No-Touch isolation procedures might prevent the dissemination of cancer cells from the primary tumor. However, these techniques are not widely accepted in laparoscopic pancreatoduodenectomy (LPD) by now. We herein present in-situ No-Touch isolation LPD with partial resection and reconstruction of the superior mesenteric vein (SMV) for pancreatic cancer after neoadjuvant therapy.

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Genetics

Investigating the Pathogenesis of MYH7 Mutation Gly823Glu in Familial Hypertrophic Cardiomyopathy using a Mouse Model
Yu Xia *1,2, Jinlin Hu *3, Xiang Li 4, Shuang Zheng 4, Ge Wang 1,2, Songtao Tan 1,2, Zengxiao Zou 1,2, Qiong Ling 2,5, Fenghua Yang 4, Xiaoping Fan 1,2
1Department of Cardiovascular Surgery, Guangdong Provincial Hospital of Traditional Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 2The Second Clinical College of Guangzhou University of Chinese Medicine, 3Department of Cardiovascular Surgery, The First Affiliated Hospital, Jinan University, 4Guangdong Provincial Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, 5Department of Anesthesiology, Guangdong Provincial Hospital of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine

Based on the familial hereditary cardiomyopathy family found in our clinical work, we created a C57BL/6N mouse model with a point mutation (G823E) at the mouse MYH7 locus through CRISPR/Cas9-mediated genome engineering to verify this mutation.

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Neuroscience

Dual-Task Stroop Paradigm for Detecting Cognitive Deficits in High-Functioning Stroke Patients
Songjun Lin 1,2, Qiang Lin 3,4,5, Biyi Zhao 3,4,6, Yongchun Jiang 3,4, Wanqi Zhuang 3,4, Delong Chen 3,4, Yajie Zhang 3,4, Aijia Chen 3,4, Qianrong Zhang 7, Yuxin Zheng 3,4, Jianjun Wang 1, Fangqiu Xu 8, Xi Qin 3,4,9, Yefeng Cai 10
1Department of Neurology and Psychology, the Fourth Clinical Medical College, Guangzhou University of Chinese Medicine, Shenzhen Traditional Chinese Medicine Hospital, 2Guangzhou University of Chinese Medicine, 3Department of Rehabilitation Medicine, The Fifth Affiliated Hospital of Guangzhou Medical University, 4Department of Rehabilitation Medicine, Guangzhou Medical University, 5Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, The Fifth Affiliated Hospital of Guangzhou Medical University, 6School of Traditional Chinese Medicine, Jinan University, 7Health College of Guangdong Pharmaceutical University, 8Department of Clinical Medicine, Guangzhou Medical University, 9Department of Rehabilitation Medicine, Panzhihua Central Hospital, 10Department of Neurology and Psychology, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine

Clinical assessment scales are notsensitive enough to cognitive dysfunction in high-functioning stroke patients. The dual-task paradigm presents advantages and potential in the assessment and cognitive training of cognitive dysfunction. The study here proposes a dual-task Stroop paradigm to identify cognitive dysfunction in high-functioning stroke patients.

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Medicine

Management of the Uncinate Process in No-Touch Laparoscopic Pancreaticoduodenectomy
Zhantao Shen 1,2, Xiang Wu 1,2, Fanxing Huang 2, Guihao Chen 1,2, Yifeng Liu 1,2, Zhimin Yu 1,2, Chunbao Zhu 1,2, Zhijian Tan 1,2, Xiaosheng Zhong 1,2
1The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 2The Second Clinical College of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou University of Chinese Medicine

Complete resection of the uncinate process and mesopancreas is one of the most important and difficult processes in laparoscopic pancreatoduodenectomy (LPD). This article presents a method for managing the uncinate process in no-touch LPD using the median-anterior and left-posterior approaches to the superior mesenteric artery (SMA).

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Medicine

Fu's Subcutaneous Needling for Knee Osteoarthritis Pain
Po-En Chiu *1,2, Zhonghua Fu *3,4, Jian Sun 4,5,6, Guan-Wei Jian 7,8, Te-Mao Li 9, Li-Wei Chou 10,11,12
1Department of Chinese Medicine, Chang Bing Show Chwan Memorial Hospital, 2Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, 3Institute of Fu's Subcutaneous Needling, Beijing University of Chinese Medicine, 4Clinical Medical College of Acupuncture & Moxibustion and Rehabilitation, Guangzhou University of Chinese Medicine, 5Second Clinical Medical College, Guangzhou University of Chinese Medicine, 6Guangdong Provincial Hospital of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 7Graduate Institute of Acupuncture Science, China Medical University, 8Department of Chinese Medicine, Sinying Hospital, Ministry of Health and Welfare, 9School of Chinese Medicine, College of Chinese Medicine, China Medical University, 10Department of Physical Medicine and Rehabilitation, China Medical University Hospital, 11Department of Physical Therapy and Graduate Institute of Rehabilitation Science, China Medical University, 12Department of Physical Medicine and Rehabilitation, Asia University Hospital, Asia University

We present a protocol for using Fu's subcutaneous needling for knee osteoarthritis pain, which combines swaying movement and reperfusion approach techniques. This protocol has great potential for future applications in myofascial pain treatment and could enhance Fu's subcutaneous needling (FSN) manipulation skills.

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