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Yangtze University

3 ARTICLES PUBLISHED IN JoVE

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Environment

Shootward Movement of CFDA Tracer Loaded in the Bottom Sink Tissues of Arabidopsis
Mengting Jiang *1, Zhuying Deng *1, Rosemary G. White 2, Tianlin Jin 1, Dacheng Liang 1
1Hubei Collaborative Innovation Center for Grain Industry, School of Agriculture, Yangtze University, 2CSIRO Agriculture

The goal of this protocol is to show how to load the CFDA into different sites of the bottom parts of Arabidopsis. We then present the resulting distribution pattern of CF in the shoots.

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Genetics

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens
Jinxia Shi 1, Yijuan Jia 1, Di Fang 1, Shidan He 1, Peng Zhang 1,2, Yushuang Guo 3, Yongli Qiao 1
1Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, 2College of Agriculture, Yangtze University, 3Laboratory of Molecular Genetics, China National Tobacco Corporation, Guizhou Institute of Tobacco Science

Here, we present a modified screening method that can be extensively used to quickly screen RNA silencing suppressors in plant pathogens.

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Cancer Research

Knockdown of FAM83A to Verify Its Role in Cervical Cancer Cell Growth and Cisplatin Sensitivity
Sisi Zhang *1, Xuemei Lin *2, Li Xiao 1, Ya Wang 3
1Department of Obstetrics and Gynecology, Jingzhou Hospital, Yangtze University, 2Department of Obstetrics and Gynaecology, Gong An County People's Hospital, 3Department of Ultrasound, Zhijiang People's Hospital

Here, we show the procedures for FAM83A knockdown; the assays to detect its effects on proliferation, migration, and invasion of cervical cancer cells; and the sensitization of these cells to cisplatin. This study provides a promising target gene for cervical cancer and a reference for further drug research.

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