Sign In

IRCCS Istituto Ortopedico Galeazzi

1 ARTICLES PUBLISHED IN JoVE

image

Biology

Setting a Successful Sorting for Extracellular Vesicle Isolation
Monica Romanò *1, Daniela Boselli *1, Enrico Ragni 2, Laura de Girolamo 2, Chiara Villa 1,3
1FRACTAL - Flow cytometry Resource, Advanced Cytometry Technical Applications Laboratory, IRCCS Ospedale San Raffaele, 2Laboratorio di Biotecnologie Applicate all'Ortopedia, IRCCS Istituto Ortopedico Galeazzi, 3Università Vita-Salute San Raffaele

This protocol provides a detailed description of sorting extracellular vesicles (EVs) released by mesenchymal stromal cells. In particular, it focuses on the instrument setting and the optimization of the sorting conditions. The goal is to sort extracellular vesicles while preserving their characteristics.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved