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Japan Women's University

2 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion
Yuno Natsume 1, Hsin-i Wen 2, Tong Zhu 2, Kazumi Itoh 1, Li Sheng 2, Kensuke Kurihara 2,3,4
1Department of Mathematical and Physical Sciences, Faculty of Science, Japan Women's University, 2Department of Bioorganization Research, Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, 3Department of Life and Coordination-Complex Molecular Science, Institute for Molecular Science, National Institutes of Natural Sciences, 4Research Center for Complex Systems Biology, The University of Tokyo

Giant vesicles containing highly packed micrometer-sized components are useful cell models. The water-in-oil emulsion centrifugation method is a simple, powerful tool for the preparation of giant vesicles with encapsulated materials.

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Biology

Establishment of a Clonal Culture of Unicellular Conjugating Algae
Yuki Tsuchikane 1, Takashi Hamaji 2, Katsumi Ota 3, Syou Kato 4
1Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, 2Department of Botany, Graduate School of Science, Kyoto University, 3Katsumi Ota Translation Office, 4Wetlands International Japan

In this article, we demonstrate the establishment of clonal cultures of unicellular conjugating algal species collected from a natural field site.

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