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Radboudumc

4 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Interfacing 3D Engineered Neuronal Cultures to Micro-Electrode Arrays: An Innovative In Vitro Experimental Model
Mariateresa Tedesco 1, Monica Frega 1,2, Sergio Martinoia 1, Mattia Pesce 3, Paolo Massobrio 1
1Department of Informatics, Bioengineering, Robotics and System Engineering (DIBRIS), University of Genova, 2Donders Institute for Brain, Cognition and Behaviour, Department of Cognitive Neuroscience, Radboud University Medical Center, 3Fondazione Istituto Italiano di Tecnologia (IIT)

In this work, a novel experimental model in which 3D neuronal cultures are coupled to planar Micro-Electrode Arrays (MEAs) is presented. 3D networks are built by seeding neurons in a scaffold made up of glass microbeads on which neurons grow and form interconnected 3D structures.

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Developmental Biology

Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays
Monica Frega *1,2, Sebastianus H. C. van Gestel *3, Katrin Linda 2,3, Jori van der Raadt 3, Jason Keller 1,2, Jon-Ruben Van Rhijn 1,2, Dirk Schubert 1,2, Cornelis A. Albers 2,3,4, Nael Nadif Kasri 1,2,3
1Department of Cognitive Neurosciences, Radboudumc, 2Donders Institute for Brain, Cognition and Behaviour, Radboud University, 3Department of Human Genetics, Radboudumc, 4Department of Molecular Developmental Biology, Radboud University

We modify and implement a previously published protocol describing the rapid, reproducible, and efficient differentiation of human induced Pluripotent Stem Cells (hiPSCs) into excitatory cortical neurons12. Specifically, our modification allows for control of neuronal cell density and use on micro-electrode arrays to measure electrophysiological properties at the network level.

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Genetics

Exploring X Chromosomal Aberrations in Ovarian Cells by Using Fluorescence In Situ Hybridization
Sapthami Nadesapillai 1, Janielle van der Velden 2, Didi Braat 1, Kathrin Fleischer 1,3, Ronald Peek 1
1Department of Obstetrics and Gynecology, Radboudumc, 2Department of Pediatrics, Radboudumc, Amalia Children’s Hospital, 3Department of Reproductive Medicine, Nij Geertgen Center for Fertility

This article presents two methods based on fluorescence in situ hybridization to determine the X chromosomal content of ovarian cells in non-grafted and grafted ovarian cortex tissue from females with X chromosomal aberrations.

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Cancer Research

Multiplex Immunohistochemical Analysis of the Spatial Immune Cell Landscape of the Tumor Microenvironment
Mark A. J. Gorris 1,2, Evgenia Martynova *1,3, Mark W. D. Sweep *1,4, Iris A. E. van der Hoorn 1,5, Shabaz Sultan 1,3, Mike J. D. E. Claassens 1, Lieke L. van der Woude 1,2,6, Kiek Verrijp 1,2,6, Carl G. Figdor 1,3, Johannes Textor 1,3, I. Jolanda M. de Vries 1
1Department of Medical BioSciences, Radboudumc, 2Division of Immunotherapy, Oncode Institute, Radboudumc, 3Data Science, Institute for Computing and Information Sciences, Radboud University, 4Department of Medical Oncology, Radboudumc, 5Department of Pulmonary Diseases, Radboudumc, 6Department of Pathology, Radboudumc

This protocol describes in detail how immune cell characterization of the tumor microenvironment using multiplex immunohistochemistry is carried out.

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