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Sao Paulo State University (UNESP)

1 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies
Claudia Neves Correa 1,2, Louise Oliveira Fiametti 1, Maria Eduarda Mazzi Esquinca 1, Leandro Mantovani de Castro 1,2
1Department of Biological and Environmental Sciences, Bioscience Institute, Sao Paulo State University (UNESP), 2Biodiversity of Coastal Environments Postgraduate Program, Bioscience Institute, Sao Paulo State University (UNESP)

This article describes a sample preparation method based on heat-inactivation to preserve endogenous peptides avoiding degradation post-mortem, followed by relative quantitation using isotopic labeling plus LC-MS.

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