Wide-field Single-photon Optical Recording in Brain Slices Using Voltage-sensitive DyeYoko Tominaga 1, Makiko Taketoshi 1, Naoko Maeda 1, Takashi Tominaga 1
1Laboratory for Neural Circuit Systems, Institute of Neuroscience, Tokushima Bunri University
We introduce a reproducible and stable optical recording method for brain slices using voltage-sensitive dye. The article describes voltage-sensitive dye staining and recording of optical signals using conventional hippocampal slice preparations.