JoVE Logo

Sign In

University of Antioquia

3 ARTICLES PUBLISHED IN JoVE

image

Immunology and Infection

Cutaneous Leishmaniasis in the Dorsal Skin of Hamsters: a Useful Model for the Screening of Antileishmanial Drugs
Sara M. Robledo 1, Lina M. Carrillo 1,2, Alejandro Daza 1, Adriana M. Restrepo 1, Diana L. Muñoz 1, Jairo Tobón 1, Javier D. Murillo 1, Anderson López 1, Carolina Ríos 1, Carol V. Mesa 1, Yulieth A. Upegui 1, Alejandro Valencia-Tobón 1, Karina Mondragón-Shem 1, Berardo RodrÍguez 2, Iván D. Vélez 1
1Program for the Study and Control of Tropical Diseases -PECET-School of Medicine, University of Antioquia, 2School of Agrarian Sciences, University of Antioquia

Optimization of the experimental hamster model for cutaneous leishmaniasis by intradermal injection of Leishmania promastigotes at the dorsal skin. This approach is useful during inoculation, follow-up, characterization of lesions, application of treatments and obtaining of clinical samples. Locomotion, search for food and water, play and social activities are preserved.

image

Bioengineering

Fabrication of a Functionalized Magnetic Bacterial Nanocellulose with Iron Oxide Nanoparticles
Sandra L. Arias 1, Akshath R. Shetty 2, Angana Senpan 3, Mónica Echeverry-Rendón 4, Lisa M. Reece 5,6, Jean Paul Allain 1,2,3,7
1Department of Bioengineering, University of Illinois at Urbana-Champaign, 2Department of Nuclear, Plasma and Radiological Engineering, University of Illinois at Urbana-Champaign, 3Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, 4Program of Study and Control of Tropical Diseases (PECET), University of Antioquia, 5Sealy Center for Vaccine Development, University of Texas Medical Branch, 6WHO Collaborating Center for Vaccine Research, Evaluation and Training on Emerging Infectious Diseases, University of Texas Medical Branch, 7Beckman Institute, University of Illinois at Urbana-Champaign

Here, we present a protocol to make a bacterial nanocellulose (BNC) magnetic for applications in damaged blood vessel reconstruction. The BNC was synthesized by G. xylinus strain. On the other hand, magnetization of the BNC was realized through in situ precipitation of Fe2+ and Fe3+ ferrous ions inside the BNC mesh.

image

Biology

Intact Short, Intermediate, and Long Skeletal Muscle Fibers Obtained by Enzymatic Dissociation of Six Hindlimb Muscles of Mice: Beyond Flexor Digitorum Brevis
Jorge L. Petro *1, Andrés F. Milán *1, Erika Arenas 1, Laura Valle 1, Valeria Hernández 1, Juan C. Calderón 1
1Physiology and Biochemistry Research Group-PHYSIS, Faculty of Medicine, University of Antioquia

We describe a protocol to obtain enzymatically dissociated fibers of different lengths and types from six muscles of adult mice: three of them already described (flexor digitorum brevis, extensor digitorum longus, soleus) and three of them successfully dissociated for the first time (extensor hallucis longus, peroneus longus, peroneus digiti quarti).

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved