The Lodz University of Technology

5 ARTICLES PUBLISHED IN JoVE

Biology

Lens Transplantation in Zebrafish and its Application in the Analysis of Eye Mutants

Yan Zhang ^1,2, Kyle McCulloch², Jarema Malicki²

¹The Second Teaching Hospital of Jilin University, ²Department of Ophthalmology, Harvard Medical School

Lens development involves interactions with other tissues. Several zebrafish eye mutants are characterized by an abnormally small lens size. Here we demonstrate a lens transplantation experiment to determine whether this phenotype is due to intrinsic causes or defective interactions with tissues that surround the lens.

Behavior

Using Gold-standard Gait Analysis Methods to Assess Experience Effects on Lower-limb Mechanics During Moderate High-heeled Jogging and Running

Yan Zhang ^1,2,3, Meizi Wang¹, Jan Awrejcewicz³, Gusztáv Fekete⁴, Feng Ren¹, Yaodong Gu^1,2

¹Faculty of Sports Science, Ningbo University, ²Research Academy of Grand Health Interdisciplinary, Ningbo University, ³Department of Automation, Biomechanics and Mechatronics, The Lodz University of Technology, ⁴Savaria Institute of Technology, Eötvös Loránd University

This study investigated lower-limb kinematics and ground reaction force (GRF) during moderate high-heeled jogging and running. Subjects were divided into groups of experienced wearers and inexperienced wearers. A three-dimensional motion analysis system with a configured force platform captured lower-limb joint movements and GRF.

Medicine

Re-Arterialized Rat Partial Liver Transplantation with an in vivo Vessel-Oriented 70% Hepatectomy

Xuehai Chen¹, Rong Yu², Ziqiang Xu³, Yan Zhang ³, Chengyang Liu⁴, Bicheng Chen^*1, Hao Jin^*3

¹Department of Surgery, The First Affiliated Hospital of Wenzhou Medical University, ²Reproductive Center, The First Affiliated Hospital of Wenzhou Medical University, ³Department of Transplantation, The First Affiliated Hospital of Wenzhou Medical University, ⁴Department of Surgery, Perelman School of Medicine at the University of Pennsylvania

Here, a protocol involving re-arterialized rat partial liver transplantation is presented. Specifically, 70% liver was resected in vivo by using an updated technique of vessel-oriented hepatectomy. The hepatic artery was reconstructed in an end-to-side manner. The cuff technique was modified to shorten the anastomosis time of the infrahepatic vena cava.

Behavior

Biomechanical Analysis Methods to Assess Professional Badminton Players' Lunge Performance

Ping Huang^1,2,3, Lin Fu¹, Yan Zhang ³, Gusztáv Fekete⁴, Feng Ren¹, Yaodong Gu^1,2

Here, we present a protocol to evaluate the differences in injury mechanisms between professional and amateur players when performing a badminton maximal right lunge movement by analyzing lower limb kinematics.

Neuroscience

Isolation and Culture of Primary Neurons and Glia from Adult Rat Urinary Bladder

Rui Wang¹, Zi-tong Huang³, Wen-kang Ren¹, Jiao Zhang¹, Yao Zhang¹, Bo Tan³, Ping Huang^1,2, Hong-ying Cao^1,2

¹School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, ²Dongguan & Guangzhou University of Chinese Medicine Cooperative Academy of Mathematical Engineering for Chinese Medicine, Guangzhou University of Chinese Medicine, ³School of Basic Medical Sciences, Guangzhou University of Chinese Medicine

This protocol attempts to establish a repeatable protocol for primary neurons and glia isolation from rat bladder for further cellular experiments.