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Shanxi Medical University

2 ARTICLES PUBLISHED IN JoVE

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JoVE Journal

Use of In Vivo Single-fiber Recording and Intact Dorsal Root Ganglion with Attached Sciatic Nerve to Examine the Mechanism of Conduction Failure
Honghui Mao *1,2, Xiuchao Wang *1,3, Wen Chen 4, FengYu Liu 5, You Wan 5, Sanjue Hu 1, Junling Xing 1,6
1Department of Neurobiology, School of Basic Medicine, Fourth Military Medical University, 2Department of Toxicology, School of Public Health, ShanXi Medical University, 3Department of Psychology, Fourth Military Medical University, 4Department of Neurobiology, School of Basic Medical Sciences, Advanced Innovation Center for Human Brain Protection, Capital Medical University, 5Neuroscience Research Institute, Key Lab for Neuroscience, Ministry of Education/National Health Commission, Peking University, 6Department of Radiation Biology, Faculty of Preventive Medicine, Fourth Military Medical University

Single-fiber recording is an effective electrophysiological technique that is applicable to the central and peripheral nervous systems. Along with the preparation of intact DRG with the attached sciatic nerve, the mechanism of conduction failure is examined. Both protocols improve the understanding of the peripheral nervous system's relationship with pain.

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Neuroscience

Handcrafted Silicone Coated Filament for Mice Middle Cerebral Artery Occlusion Models
Min Zhang 1,2, Yitong Yuan 3, Ruochen Du 3, Chunli Wen 1, Shiqin Lin 2, Yaqiong Zhang 2, Zhenyu Xiang 4, Fengyun Hu 1, Chunfang Wang 3
1Department of Neurology, Fifth Hospital of Shanxi Medical University, Shanxi Provincial People's Hospital, 2Department of Neurology, Taiyuan Central Hospital, Shan Xi Medical University, 3Experimental Animal Center, Shanxi Medical University, 4Collaborative Innovation Center for Molecular Imaging of Precision Medicine, Shan Xi Medical University

This protocol describes a straightforward method for creating coated filaments for the middle cerebral artery occlusion (MCAO) model in mice using silicone, nylon sutures, and syringe needles. This method allows for the production of filaments with a consistent diameter and various silicone wrapping lengths tailored to experimental needs.

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