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University of Basel Children's Hospital

4 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
Xinzhou Zhu 1, Andrea Zelmer 1, Sven Wellmann 1,2
1University Children's Hospital Basel (UKBB), University of Basel, 2Department of Clinical Research, University of Basel

Protein-protein interactions can occur in both the nucleus and the cytoplasm of a cell. To investigate these interactions, traditional co-immunoprecipitation and modern proximity ligation assay are applied. In this study, we compare these two methods to visualize the distribution of NF90-RBM3 interactions in the nucleus and the cytoplasm.

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Medicine

Electrophysiological Measurement of Noxious-evoked Brain Activity in Neonates Using a Flat-tip Probe Coupled to Electroencephalography
Noemi Klarer *1, Hanna Rickenbacher *1, Severin Kasser 1, Antoinette Depoorter 2, Sven Wellmann 1
1Division of Neonatology, University of Basel Children's Hospital, 2Divison of Neuropediatrics and Developmental Medicine, University of Basel Children's Hospital

Measuring pain in non-verbal patients is a challenge. In this study we combine EEG recording with stimulation using a flat-tip probe to detect noxious-evoked brain activity in an objective manner.

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Behavior

An Objective and Child-friendly Assessment of Arm Function by Using a 3-D Sensor
Xing Chen 1, Detlef Wolf 1, Juliane Siebourg-Polster 2, Christian Czech 3, Ulrike Bonati 4,5, Dirk Fischer 4,5, Omar Khwaja 6, Martin Strahm 1
1Data Science, Roche Pharmaceutical Research and Early Development Informatics, Roche Innovation Center Basel, F. Hoffmann-La Roche, Ltd., 2Translational Technologies and Bioinformatics, Pharmaceutical Sciences, Roche Pharmaceutical Research and Early Development, Roche Innovation Center Basel, F. Hoffmann-La Roche, Ltd., 3Biomarker Experimental Medicine, Neuroscience, Roche Pharmaceutical Research and Early Development, Roche Innovation Center Basel, F. Hoffmann-La Roche, Ltd., 4Division of Neuropediatrics, University of Basel Children's Hospital, 5Department of Neurology, University of Basel Hospital, 6Translational Medicine, Neuroscience and Rare Diseases, Roche Pharmaceutical Research and Early Development, Roche Innovation Center Basel, F. Hoffmann-La Roche, Ltd.

An objective measure of muscle functions is challenging especially in children. Based on a commercially available digital 3-D sensor, a child-friendly gaming test was developed to assess upper limb function for clinical trials.

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Medicine

Measurements of Motor Function and Other Clinical Outcome Parameters in Ambulant Children with Duchenne Muscular Dystrophy
Sara Nagy 1,2, Simone Schmidt 1,2, Patricia Hafner 1,3, Andrea Klein 1,4,5, Daniela Rubino-Nacht 1, Vanya Gocheva 1, Oliver Bieri 6, Carole Vuillerot 7, Ulrike Bonati 1,2, Dirk Fischer 1,2,3
1Division of Pediatric Neurology, University Children's Hospital Basel (UKBB), University of Basel, 2Department of Neurology, University Hospital Basel, University of Basel, 3Division of Neurology, University Clinic of Medicine, Cantonal Hospital Baselland, Bruderholz, 4Division of Pediatric Neurology, CHUV, 5Division of Neuropaediatrics, Inselspital, University Children's Hospital Bern, 6Department of Radiology, Division of Radiological Physics, University Hospital Basel, University of Basel, 7Hospices Civils de Lyon, Hôpital Femme-Mère-Enfant, L'Escale, Service de Médecine Physique et de Réadaptation Pédiatrique

The aim of this study is to present the most reliable clinical outcome measures and their correlations with quantitative muscle MRI in ambulant patients with Duchenne muscular dystrophy.

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