Morgagni-Pierantoni Hospital

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

Cell-Free DNA Integrity Analysis in Urine Samples

Valentina Casadio¹, Samanta Salvi¹, Filippo Martignano¹, Roberta Gunelli², Sara Ravaioli¹, Daniele Calistri¹

¹Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, ²Department of Urology, Morgagni-Pierantoni Hospital

A method for analyzing DNA integrity in the cell-free supernatant fraction of urine samples is proposed. The method is suitable for early detection of urological malignancies and has proven accurate for the early diagnosis of bladder cancer.

Cancer Research

Establishment of a Primary Culture of Patient-derived Soft Tissue Sarcoma

Alessandro De Vita¹, Laura Mercatali¹, Giacomo Miserocchi¹, Chiara Liverani¹, Chiara Spadazzi¹, Federica Recine¹, Alberto Bongiovanni¹, Federica Pieri², Davide Cavaliere³, Valentina Fausti¹, Dino Amadori¹, Toni Ibrahim¹

¹Osteoncology and Rare Tumors Center, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, ²Pathology Unit, Morgagni-Pierantoni Hospital, ³Unit of Surgery and Advanced Oncologic Therapies, Morgagni-Pierantoni Hospital

The following protocol focuses on the establishment of a primary culture of patient-derived soft tissue sarcoma (STS). This model could help us to better understand the molecular background and pharmacological profile of these rare malignancies and could represent a starting point for further research aimed at improving STS management.