University of Veterinary Medicine Vienna

2 ARTICLES PUBLISHED IN JoVE

Medicine

Human Internal Mammary Artery (IMA) Transplantation and Stenting: A Human Model to Study the Development of In-Stent Restenosis

Xiaoqin Hua^1,2, Tobias Deuse^1,2, Evangelos D. Michelakis³, Alois Haromy³, Phil S. Tsao⁴, Lars Maegdefessel⁴, Reinhold G. Erben⁵, Claudia Bergow⁵, Boris B. Behnisch⁶, Hermann Reichenspurner^1,2, Robert C. Robbins⁷, Sonja Schrepfer^1,2,7

¹University Heart Center Hamburg, TSI-Lab, Germany, ²Cardiovascular Research Center, University of Hamburg, ³Department of Medicine, Cardiology Division, Pulmonary Hypertension Program, University of Alberta, ⁴Department of Medicine, Stanford University School of Medicine , ⁵Department of Biomedical Sciences, Institute of Physiology, Pathophysiology, and Biophysics, University of Veterinary Medicine, Vienna, ⁶Translumina GmbH, Hechingen, ⁷Department of Cardiothoracic Surgery, Stanford University School of Medicine

This video shows a model to study the development of intimal hyperplasia after stent deployment using a human vessel (IMA) in an immunodeficient rat model.

Biology

A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones

Ana Marek¹, Christiane Schüler¹, María Satué¹, Barbara Haigl¹, Reinhold G. Erben¹

¹Department of Biomedical Research, University of Veterinary Medicine Vienna

A laser capture microdissection (LCM) protocol was developed to obtain sufficient quantity of high-quality RNA for gene expression analysis in bone cells. The current study focusses on mouse femur sections. However, the LCM protocol reported here can be used to study gene expression in cells of any hard tissue.