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Ohio State University Wexner Medical Center

10 ARTICLES PUBLISHED IN JoVE

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Medicine

High-frequency High-resolution Echocardiography: First Evidence on Non-invasive Repeated Measure of Myocardial Strain, Contractility, and Mitral Regurgitation in the Ischemia-reperfused Murine Heart
Surya C. Gnyawali 1,2, Sashwati Roy 1,2, Jason Driggs 1,2, Savita Khanna 1,2, Thomas Ryan 2,3, Chandan K. Sen 2
1Department of Surgery, The Ohio State University, 2Heart and Lung Research Institute, The Ohio State University, 3Department of Cardiovascular Medicine, The Ohio State University

High frequency Doppler ultrasound is a novel technology for assessing regional myocardial function. This work presents first evidence demonstrating applicability of this versatile imaging platform for the repeated measure of myocardial strain, dp/dt, and mitral regurgitation in the ischemia-reperfused (IR) murine heart.

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Medicine

Dual-mode Imaging of Cutaneous Tissue Oxygenation and Vascular Function
Ronald X. Xu 1, Kun Huang 2, Ruogu Qin 1, Jiwei Huang 1, Jeff S. Xu 1, Liya Ding 2, Urmila S. Gnyawali 3, Gayle M. Gordillo 3, Surya C. Gnyawali 3,4, Chandan K. Sen 3
1Department of Biomedical Engineering, The Ohio State University, 2Department of Biomedical Informatics, The Ohio State University, 3Comprehensive Wound Center, The Ohio State University, 4Department of Surgery, The Ohio State University

A dual-mode imaging system was developed for non-contact assessment of cutaneous tissue oxygenation and vascular function.

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Medicine

Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program
Kevin Nelson 1,2, Christopher Bobba 1,2, Emre Eren 3, Tyler Spata 4, Malak Tadres 2, Don Hayes, Jr. 5,6, Sylvester M. Black 3,7, Samir Ghadiali *1,2,3, Bryan A. Whitson *2,3,4
1Department of Biomedical Engineering, Ohio State University Wexner Medical Center, 2Davis Heart & Lung Research Institute, Ohio State University Wexner Medical Center, 3The Collaboration for Organ Perfusion, Protection, Engineering and Regeneration (COPPER) Laboratory, Ohio State University Wexner Medical Center, 4Division of Cardiac Surgery, Department of Surgery, Ohio State University Wexner Medical Center, 5Departments of Pediatrics and Internal Medicine, Ohio State University, 6Advanced Lung Disease Program, Lung and Heart-Lung Transplant Programs, Nationwide Children's Hospital, 7Division of Transplantation, Department of Surgery, Ohio State University Wexner Medical Center

Ex-Vivo Lung Perfusion (EVLP) has allowed lung transplantation in humans to become more readily available by enabling the ability to assess organs and expand the donor pool. Here, we describe the development of a rat EVLP program and refinements that allow for a reproducible model for future expansion.

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Medicine

Method of Direct Segmental Intra-hepatic Delivery Using a Rat Liver Hilar Clamp Model
Eliza W. Beal 1, Curtis Dumond 1, Jung-Lye Kim 1, Khalid Mumtaz 1, Don Hayes Jr. 1, Ken Washburn 1, Bryan A. Whitson 1, Sylvester M. Black 1
1Collaboration for Organ Perfusion, Protection, Engineering and Regeneration (COPPER) Lab, Division of Transplant, Department of Surgery, Comprehensive Transplant Center, The Ohio State University Wexner Medical Center

A unique rat liver hilar clamp model was developed for studying the impact of pharmacologic molecules in ameliorating ischemia-reperfusion injury. This model includes direct cannulation of the portal supply to the ischemic liver segment via a branch of the portal vein, allowing for direct hepatic delivery.

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Environment

Preparation of Authigenic Pyrite from Methane-bearing Sediments for In Situ Sulfur Isotope Analysis Using SIMS
Zhiyong Lin 1,3, Xiaoming Sun 1,2,3,4, Jörn Peckmann 5, Yang Lu 2,3, Harald Strauss 6, Li Xu 2,3, Hongfeng Lu 7, Barbara M.A. Teichert 6
1School of Earth Sciences and Engineering, Sun Yat-sen University, 2School of Marine Sciences, Sun Yat-sen University, 3Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, 4South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, 5Institut für Geologie, Universität Hamburg, 6Institut für Geologie und Paläontologie, Westfälische Wilhelms-Universität Münster, 7Guangzhou Marine Geological Survey

Analyses of the sulfur isotopic composition (δ34S) of pyrite from methane-bearing sediments have typically focused on bulk samples. Here, we applied secondary ion mass spectroscopy to analyze the δ34S values of various pyrite generations to understand the diagenetic history of pyritization.

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Medicine

A Small Animal Model of Ex Vivo Normothermic Liver Perfusion
Eliza W. Beal 1,2, Curtis Dumond 1, Jung-Lye Kim 1,2, Clifford Akateh 1,2, Emre Eren 1, Katelyn Maynard 1, Chandan K. Sen 3, Jay L. Zweier 4, Kenneth Washburn 2, Bryan A. Whitson 1,3, Sylvester M. Black 1,2
1Collaboration for Organ Perfusion, Protection, Engineering and Regeneration (COPPER) Lab, Division of Transplant, Department of Surgery, Comprehensive Transplant Center, Ohio State University Wexner Medical Center, 2Department of Surgery, Division of Transplant, Ohio State University Wexner Medical Center, 3Department of Surgery, Division of CardioThoracic Surgery, Ohio State University Wexner Medical Center, 4Department of Medicine, Ohio State University Wexner Medical Center

There is a significant liver donor shortage, and criteria for liver donors have been expanded. Normothermic ex vivo liver perfusion (NEVLP) has been developed to evaluate and modify organ function. This study demonstrates a rat model of NEVLP and tests the ability of pegylated-catalase, to mitigate liver preservation injury.

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Medicine

Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice
Li Xu 1, Yandi Gao 1, Yeh Siang Lau 1, Renzhi Han 1
1Department of Surgery, Davis Heart and Lung Research Institute, Biomedical Sciences Graduate Program, Biophysics Graduate Program, Ohio State University Wexner Medical Center

Here we provide a detailed protocol to carry out in vivo cardiac gene editing in mice using recombinant Adeno-Associated Virus(rAAV)-mediated delivery of CRISPR. This protocol offers a promising therapeutic strategy to treat dystrophic cardiomyopathy in Duchenne muscular dystrophy and can be used to generate cardiac-specific knockout in postnatal mice.

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Medicine

Robotic Radical Cystectomy, Pelvic Lymph Node Dissection, and Intracorporeal Ileal Conduit Urinary Diversion
Stephanie A. Stillings 1, Debasish Sundi 1
1Department of Urology, Ohio State University Wexner Medical Center

This paper describes robotic radical cystectomy, pelvic lymph node dissection, and intracorporeal ileal conduit urinary diversion.

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Biology

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue
Daria Barwinska 1, Michael J. Ferkowicz 1, Ying-Hua Cheng 1, Seth Winfree 1,2, Kenneth W. Dunn 1, Katherine J. Kelly 1, Timothy A. Sutton 1, Brad H. Rovin 3, Samir V. Parikh 3, Carrie L. Phillips 4, Pierre C. Dagher 1, Tarek M. El-Achkar 1, Michael T. Eadon 1,
1Department of Medicine, Indiana University School of Medicine, 2Department of Cellular & Integrative Physiology, Indiana University School of Medicine, 3Division of Nephrology, Department of Medicine, Ohio State University Wexner Medical Center, 4Division of Pathology, Indiana University School of Medicine

We describe a protocol for laser microdissection of sub-segments of the human kidney, including the glomerulus, proximal tubule, thick ascending limb, collecting duct and interstitium. The RNA is then isolated from the obtained compartments and RNA sequencing is carried out to determine changes in the transcriptomic signature within each sub-segment.

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Neuroscience

Lineage Tracing of Inducible Fluorescently-Labeled Stem Cells in the Adult Mouse Brain
Gabriel S. Jensen 1,2, Jake W. Willows 2, David T. Breault 3, Kristy L. Townsend 1,2
1Graduate School of Biomedical Science and Engineering, University of Maine, 2Department of Neurosurgery, Ohio State University Wexner Medical Center, 3Boston Children’s Hospital, Harvard Medical School

The ability to permanently mark stem cells and their progeny with a fluorophore using an inducible transgenic lineage tracing mouse line allows for spatial and temporal analysis of activation, proliferation, migration, and/or differentiation in vivo. Lineage tracing can reveal novel information about lineage commitment, response to intervention(s), and multipotency.

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