Sign In

Allen Institute for Cell Science

1 ARTICLES PUBLISHED IN JoVE

image

Genetics

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
Amanda Haupt 1, Tanya Grancharova 1, Joy Arakaki 1, Margaret A. Fuqua 1, Brock Roberts 1, Ruwanthi N. Gunawardane 1
1Allen Institute for Cell Science

Described here is a protocol for tagging endogenously expressed proteins with fluorescent tags in human induced pluripotent stem cells using CRISPR/Cas9. Putatively edited cells are enriched by fluorescence activated cell sorting and clonal cell lines are generated.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved