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Xona Microfluidics, LLC

5 ARTICLES PUBLISHED IN JoVE

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Biology

Fabrication of a Microfluidic Device for the Compartmentalization of Neuron Soma and Axons
Joseph Harris 1, Hyuna Lee 1, Behrad Vahidi 1, Christina Tu 2, David Cribbs 3, Noo Li Jeon 1, Carl Cotman 3
1Department of Biomedical Engineering, University of California, Irvine (UCI), 2Stem Cell Research Center, University of California, Irvine (UCI), 3Institute for Brain Aging and Dementia, University of California, Irvine (UCI)

In this video we demonstrate the technique of soft lithography with polydimethyl siloxane (PDMS) which we use to farbricate a microfluidic device for culturing neurons.

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Biology

Preparing E18 Cortical Rat Neurons for Compartmentalization in a Microfluidic Device
Joseph Harris 1, Hyuna Lee 1, Christina Tu Tu 2, David Cribbs 3, Carl Cotman 3, Noo Li Jeon 1
1Department of Biomedical Engineering, University of California, Irvine (UCI), 2Stem Cell Research Center, University of California, Irvine (UCI), 3Institute for Brain Aging and Dementia, University of California, Irvine (UCI)

In this video we demonstrate the preparation of E18 Cortical Rat Neurons.

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Biology

Non-plasma Bonding of PDMS for Inexpensive Fabrication of Microfluidic Devices
Joseph Harris 1, Hyuna Lee 1, Behrad Vahidi 1, Cristina Tu 2, David Cribbs 3, Carl Cotman 3, Noo Li Jeon 1
1Department of Biomedical Engineering, University of California, Irvine (UCI), 2Stem Cell Research Center, University of California, Irvine (UCI), 3Institute for Brain Aging and Dementia, University of California, Irvine (UCI)

In this video we demonstrate how to use the neuron microfluidic device without plasma bonding.

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Neuroscience

Use of Pre-Assembled Plastic Microfluidic Chips for Compartmentalizing Primary Murine Neurons
Tharkika Nagendran 1,2, Valerie Poole 3, Joseph Harris 3, Anne Marion Taylor 1,2,3
1UNC Neuroscience Center, 2UNC/NC State Joint Department of Biomedical Engineering, UNC, 3Xona Microfluidics, LLC

This protocol describes the use of plastic chips to culture and compartmentalize primary murine neurons. These chips are preassembled, user-friendly, and compatible with high-resolution, live, and fluorescence imaging. This protocol describes how to plate rat hippocampal neurons within these chips and perform fluidic isolation, axotomy and immunostaining.

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Neuroscience

Compartmentalization of Human Stem Cell-Derived Neurons within Pre-Assembled Plastic Microfluidic Chips
Smita R. Paranjape 1,2, Tharkika Nagendran 1,2, Valerie Poole 3, Joseph Harris 3, Anne Marion Taylor 1,2,3
1UNC Neuroscience Center, 2UNC/NC State Joint Department of Biomedical Engineering, 3Xona Microfluidics, LLC

This protocol demonstrates the use of compartmentalized microfluidic chips, injection molded in a cyclic olefin copolymer to cultured neurons differentiated from human stem cells. These chips are preassembled and easier-to-use than traditional compartmentalized poly(dimethylsiloxane) devices. Multiple common experimental paradigms are described here, including viral labeling, fluidic isolation, axotomy, and immunostaining.

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