High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis (QDB)Xiaoying Qi *1, Yunyun Zhang *2, Yuan Zhang 1, Tianhui Ni 3, Wenfeng Zhang 2, Chunhua Yang 1, Jia Mi 1,4, Jiandi Zhang 2,3, Geng Tian 1
1Medicine and Pharmacy Research Center, Binzhou Medical University, 2Yantai Zestern Biotechnique Co. LTD, 3Precision Medicine research center, Binzhou Medical University, 4Department of Chemistry - BMC, Uppsala University
Here we demonstrate a detailed process of quantitative dot blot analysis (QDB) by determining the absolute content of a targeted protein, capping actin protein, gelsolin-like (CAPG), in three different mouse tissues. We demonstrate a high throughput, convenient, quantitative immunoblot technique for biomarker validation at the cellular and tissue level.
