University Hospital of Erlangen

4 ARTICLES PUBLISHED IN JoVE

JoVE Core

Dynamic Adhesion Assay for the Functional Analysis of Anti-adhesion Therapies in Inflammatory Bowel Disease

Emily Becker¹, Sebastian Schramm¹, Marie-Theres Binder¹, Clarissa Allner¹, Maximilian Wiendl¹, Clemens Neufert¹, Imke Atreya¹, Markus Neurath¹, Sebastian Zundler¹

¹Department of Medicine 1, University of Erlangen-Nuremberg

Dynamic adhesion of immune cells to the vessel wall is a prerequisite for gut homing. Here, we present a protocol for a functional in vitro assay for the impact analysis of anti-integrin antibodies, chemokines or other factors on the dynamic cell adhesion of human cells using addressin-coated capillaries.

Immunology and Infection

Induction of Intestinal Graft-versus-host Disease and Its Mini-endoscopic Assessment in Live Mice

Vera Buchele^1,3, Maike Büttner-Herold², Tina Vogler^1,3, Markus F. Neurath^1,3, Kai Hildner^1,3

¹Department of Internal Medicine 1, University Erlangen-Nürnberg, University Hospital Erlangen, ²Institute of Pathology, Department of Nephropathology, University Hospital Erlangen, ³Deutsches Zentrum Immuntherapie (DZI), University Hospital Erlangen

Here, we present a protocol that describes allogeneic hematopoietic stem cell transplantation and allows repetitive mini-endoscopic evaluations of the distal colon in situ for the presence, characteristics, and severity of colonic inflammation within live mice suffering from intestinal graft-versus-host disease.

Medicine

Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy

Anja Schulz-Kuhnt¹, Sebastian Zundler¹, Anika Grüneboom², Clemens Neufert¹, Stefan Wirtz¹, Markus F. Neurath¹, Imke Atreya¹

¹Department of Medicine 1, University Hospital of Erlangen, ²Department of Medicine 3, University Hospital of Erlangen

The here introduced protocol allows characterization of the lung homing capacity of primary human lymphocytes under in vivo inflammatory conditions. Pulmonary infiltration of adoptively transferred human immune cells in a mouse model of allergic inflammation can be imaged and quantified by light-sheet fluorescence microscopy of chemically cleared lung tissue.

Immunology and Infection

An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance

Luz DC. Martínez-Sánchez^*1, Rashmita Pradhan^*1, Phuong A. Ngo¹, Lena Erkert¹, Lukas S. Becker¹, Alastair J. Watson², Imke Atreya^*1, Markus F. Neurath^*1, Rocío López-Posadas^*1

¹Medical Clinic 1, University of Erlangen-Nuremberg, University Hospital of Erlangen, ²Norwich Medical School, University of East Anglia, Norwich Research Park

Taking advantage of intravital microscopy, the method presented here enables real-time visualization of intestinal epithelial cell shedding in living animals. Therefore, topically stained intestinal mucosa (acriflavine and rhodamineB-dextran) of anesthetized mice is imaged up to single-cell resolution using confocal microscopy.