JoVE Logo

Sign In

Hunan University of Science and Engineering

2 ARTICLES PUBLISHED IN JoVE

image

Immunology and Infection

Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis
T. Ryan Withers 1, Yeshi Yin 1, Hongwei D. Yu 1
1Department of Biochemistry and Microbiology, Joan C. Edwards School of Medicine, Marshall University

Here we describe a protocol using the mini-himar1 mariner transposon-mediated mutagenesis for generating a high-density insertion mutant library to screen, isolate and identify novel alginate regulators in the prototypic Pseudomonas aeruginosa strain PAO1.

image

Immunology and Infection

Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems
Yunfei Hu 1, Huahai Chen 1, Ping Li 2, Baiyuan Li 1, Linyan Cao 1, Changhui Zhao 1, Qing Gu 2, Yeshi Yin 1
1Key Laboratory of Comprehensive Utilization of Advantage Plants Resources in Hunan South, College of Chemistry and Bioengineering, Hunan University of Science and Engineering, 2College of Food and Biological Engineering, Zhejiang Gongshang University

Described here is a protocol to investigate the interactions between endobiotics and human gut microbiota using in vitro batch fermentation systems.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved