BrainNow Research Institute

2 ARTICLES PUBLISHED IN JoVE

Biology

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Lei Zhao^*1, Jeffrey R. Whiteaker^*1, Matthew E. Pope², Eric Kuhn³, Angela Jackson⁴, N. Leigh Anderson⁵, Terry W. Pearson², Steven A. Carr³, Amanda G. Paulovich¹

¹Clinical Research Division, Fred Hutchinson Cancer Research Center - FHCRC, ²Department of Biochemistry and Microbiology, University of Victoria, ³Broad Institute of MIT and Harvard, ⁴Genome BC Proteomics Centre, University of Victoria, ⁵Plasma Proteome Institute

Stable Isotope Standards and Capture by Anti-Peptide Antibodies (SISCAPA) couples affinity enrichment of peptides with stable isotope dilution mass spectrometry (MRM-MS) to provide quantitative measurement of peptides as surrogates for their respective proteins. Here we describe the protocol using magnetic particles in a partially automated format.

Behavior

Brain Infarct Segmentation and Registration on MRI or CT for Lesion-symptom Mapping

J. Matthijs Biesbroek¹, Hugo J. Kuijf², Nick A. Weaver¹, Lei Zhao³, Marco Duering⁴, , Geert Jan Biessels¹

¹Department of Neurology and Neurosurgery, UMC Utrecht Brain Center, University Medical Center Utrecht, Utrecht University, ²Image Sciences Institute, University Medical Center Utrecht, ³BrainNow Research Institute, ⁴Institute for Stroke and Dementia Research, University Hospital, LMU Munich

Provided here is a practical tutorial for an open-access, standardized image processing pipeline for the purpose of lesion-symptom mapping. A step-by-step walkthrough is provided for each processing step, from manual infarct segmentation on CT/MRI to subsequent registration to standard space, along with practical recommendations and illustrations with exemplary cases.