Laser Capture Microdissection on Surgical Tissues to Identify Aberrant Gene Expression in Impaired Wound Healing in Type 2 DiabetesRachael Williams *1, Irene Castellano-Pelicena *1,2, Aaiad H.A Al-Rikabi 1,3, Stephen K. Sikkink 1, Richard Baker 1, Kirsten Riches-Suman 1, M Julie Thornton 1
1Centre for Skin Sciences, University of Bradford, 2The Charles Institute of Dermatology, School of Medicine, University College Dublin, 3University of Baghdad
This technique provides a guide to inflicting ex vivo wounds, performing laser capture microdissection and quantifying changes in gene expression related to poor wound healing processes in diabetes using clinically relevant human tissue.