Economic Crop Research Institute Sichuan Academy of Agriculture Sciences

2 ARTICLES PUBLISHED IN JoVE

Medicine

Optimized Analysis of In Vivo and In Vitro Hepatic Steatosis

Aoyuan Cui¹, Zhimin Hu¹, Yamei Han¹, Yi Yang², Yu Li¹

¹CAS Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Chinese Academy of Sciences, ²Suzhou Niumag Analytical Instrument Corporation

Here, optimized methods to generate in vivo and in vitro models of hepatic steatosis and to analyze the steatotic phenotypes and related physiological parameters are described.

Genetics

Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat (Fagopyrum tataricum)

Yaolei Mi^1,2, Zhihui Zhu³, Guangtao Qian³, Yu Li⁴, Xiangxiao Meng¹, Jianping Xue³, Qingfu Chen⁵, Wei Sun¹, Yuhua Shi^1,2

¹Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, ²Artemisinin Research Center, China Academy of Chinese Medical Sciences, ³College of Life Science, Huaibei Normal University, ⁴Economic Crop Research Institute Sichuan Academy of Agriculture Sciences, ⁵Research Center of Buckwheat Industry Technology, Guizhou Normal University

We describe a method of inducing hairy roots by Agrobacterium rhizogenes-mediated transformation in Tartary buckwheat (Fagopyrum tataricum). This can be used to investigate gene functions and production of secondary metabolites in Tartary buckwheat, be adopted for any genetic transformation, or used for other medicinal plants after improvement.