Liver injuries are accompanied by progenitor cell expansion that represents a heterogeneous cell population. Novel classification of this cellular compartment allows for the distinguishing of multiple subsets. The method described here illustrates the flow cytometry analysis and high purity isolation of various subsets that can be used for further assays.
Here, we present a protocol for the development of 3D conjunctival and uveal melanoma spheroids and the use of hand-held customized electrodes for in vitro electrochemotherapy of 3D spheroids in a culture well. This offers new perspectives in the use of electrochemotherapy in ocular melanoma treatment.
We describe a clinical algorithm, based on decades of front-line experience of diagnosis and surgical treatment of human Brucellar spondylodiscitis in the largest medical center of the China's Xinjiang Pastoral Area.
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