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Saarland University Medical Center

3 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination
Henrike Julich-Haertel 1, Marina Tiwari 1, Christina Mehrfeld 1, Elmar Krause 2, Miroslaw Kornek 1, Veronika Lukacs-Kornek 1
1Department of Medicine II, Saarland University Medical Center, 2Department of Physiology, Center for Integrative Physiology and Molecular Medicine (CIPMM), University of Saarland

Liver injuries are accompanied by progenitor cell expansion that represents a heterogeneous cell population. Novel classification of this cellular compartment allows for the distinguishing of multiple subsets. The method described here illustrates the flow cytometry analysis and high purity isolation of various subsets that can be used for further assays.

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Medicine

Electrochemotherapy in 3D Ocular Melanoma Spheroids using a Customized Electrode
Miltiadis Fiorentzis 1,2, Arne Viestenz 1, Berthold Seitz 3, Sarah E Coupland 4, Joana Heinzelmann 1
1Department of Ophthalmology, University Hospital Halle (Saale), Martin-Luther University Halle-Wittenberg, 2Department of Ophthalmology, University Hospital Essen, Duisburg Essen University, 3Department of Ophthalmology, Saarland University Medical Center, 4Liverpool Ocular Oncology Research Group, Department of Molecular and Clinical Cancer Medicine, Institute of Translational Medicine, University of Liverpool

Here, we present a protocol for the development of 3D conjunctival and uveal melanoma spheroids and the use of hand-held customized electrodes for in vitro electrochemotherapy of 3D spheroids in a culture well. This offers new perspectives in the use of electrochemotherapy in ocular melanoma treatment.

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Medicine

Diagnosis and Surgical Treatment of Human Brucellar Spondylodiscitis
Yakefu Abulizi *1, Xiaoyu Cai *1,2,3, Tao Xu 1, Chuanhui Xun 1, Weibin Sheng 1, Liang Gao 2,3, Maierdan Maimaiti 1
1Department of Spine Surgery, First Affiliated Hospital of Xinjiang Medical University, 2Center of Experimental Orthopaedics, Saarland University Medical Center, 3Sino Euro Orthopaedics Network (SEON)

We describe a clinical algorithm, based on decades of front-line experience of diagnosis and surgical treatment of human Brucellar spondylodiscitis in the largest medical center of the China's Xinjiang Pastoral Area.

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