Kansas City University

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Use of Capillary Electrophoresis Immunoassay to Search for Potential Biomarkers of Amyotrophic Lateral Sclerosis in Human Platelets

Jessica M. Sage¹, LaSharice Hall², April McVey³, Richard J. Barohn³, Jeffrey M. Statland³, Omar Jawdat³, Mazen M. Dimachkie³, Abdulbaki Agbas¹

¹Department of Basic Sciences, Kansas City University of Medicine and Biosciences, ²Rice University School of Medicine, ³University of Kansas Medical Center

Blood-based biomarkers for neurodegenerative diseases are essential for implementing large-scale clinical studies. A reliable and validated blood test should require a small sample volume as well as be a less invasive sampling method, affordable, and reproducible. This paper demonstrates that high-throughput capillary electrophoresis immunoassay satisfies criteria for potential biomarker development.

Biochemistry

Measuring Mitochondrial Electron Transfer Complexes in Previously Frozen Cardiac Tissue from the Offspring of Sow: A Model to Assess Exercise-Induced Mitochondrial Bioenergetics Changes

Daniel Barrera¹, Sierra Upton², Megan Rauch³, Tara Notarianni⁴, Ki Suk Eum⁵, Megan Liberty⁶, Sarmila Venkoba Sah⁷, Robert Liu⁸, Sean Newcomer⁹, Linda E. May¹⁰, Emre Agbas¹¹, Jessica Sage¹², Edina Kosa⁷, Abdulbaki Agbas^7,13

¹AdventHelath, ²University of Illinois Chicago School of Public Health, ³Lincoln Memorial University, ⁴Children’s Mercy Hospital, ⁵Scripps Clinic, ⁶Cincinnati Children’s Hospital Medical Center, ⁷Kansas City University, ⁸Roblex Rex Veterans Affairs Medical Center, ⁹California State University San Marcos, ¹⁰East Carolina University, ¹¹Massachusetts Institute of Technology, ¹²Boehringer Ingelheim Norway KS, ¹³Heartland Center for Mitochondrial Medicine

Preparation of mitochondria-enriched samples from previously frozen archived solid tissues allowed the investigators to perform both functional and analytical assessments of mitochondria in various experimental modalities. This study demonstrates how to prepare mitochondria-enriched preparations from frozen heart tissue and perform analytical assessments of mitochondria.