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Kansas State University College of Veterinary Medicine

1 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Improving Reproducibility to Meet Minimal Information for Studies of Extracellular Vesicles 2018 Guidelines in Nanoparticle Tracking Analysis
Orman L. Snyder 1, Alexander W. Campbell 2, Lane K. Christenson 2, Mark L. Weiss 1
1Department of Anatomy and Physiology, Kansas State University College of Veterinary Medicine, 2Department of Molecular and Integrative Physiology, University of Kansas Medical Center

Nanoparticle tracking analysis (NTA) is a widely used method to characterize extracellular vesicles. This paper highlights NTA experimental parameters and controls plus a uniform method of analysis and characterization of samples and diluents necessary to supplement the guidelines proposed by MISEV2018 and EV-TRACK for reproducibility between laboratories.

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