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4 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

High Throughput Co-culture Assays for the Investigation of Microbial Interactions
Mia I. Temkin 1, Caitlin M. Carlson 1, Aaron L. Stubbendieck 2, Cameron R. Currie 1, Reed M. Stubbendieck 1
1Department of Bacteriology, University of Wisconsin-Madison, 2Independent Scholar

The co-culture interaction assays presented in this protocol are inexpensive, high throughput, and simple. These assays can be used to observe microbial interactions in co-culture, identify interaction patterns, and characterize the inhibitory potential of a microbial strain of interest against human and environmental pathogens.

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Medicine

Developing and Testing Methylated Nano-Structured Dipeptides that Inhibit Src Kinase Activity In Vitro for Anti-Cancer Applications
Alaa F. Nahhas 1, Alrayan F. Nahhas 2, Thomas J. Webster 3,4,5
1Biochemistry Department, College of Science, King Abdulaziz University, 2Independent Scholar, 3School of Health Sciences and Biomedical Engineering, Hebei University of Technology, 4School of Engineering, Saveetha University, 5Programa de Pós-graduação em Ciência e Engenharia dos Materiais, UFPI

Presented here is a protocol for designing and testing dipeptides that can inhibit Src kinase enzyme activity using acellular and cellular assays for anti-cancer applications. The peptides formulated here (W-RCH3, WCH3-RCH3, and W-R(CH3)2) inhibited Src kinase with IC50 values of 510 nM, 916 nM, and 1 µM, respectively.

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Environment

Dendrochronological Dating and Provenancing of String Instruments
Katarina Čufar 1, Blaž Demšar 2, Micha Beuting 2, Angela Balzano 1, Nina Škrk 1, Luka Krže 1, Maks Merela 1
1Department of Wood Science and Technology, Biotechnical Faculty, University of Ljubljana, 2Independent Scholar

The dendrochronological analysis of a stringed instrument requires inspecting the top plate, measuring the tree ring widths, establishing the chronology of the instrument, and dating through determining the end date-the year of the formation of the most recent tree ring.

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Biology

Streamlined Intravital Imaging Approach for Long-Term Monitoring of Epithelial Tissue Dynamics on an Inverted Confocal Microscope
Michael Hamersky IV 1, Khushi Tekale 1, L. Matthew Winfree 2, Matthew J. M. Rowan 1,3, Lindsey Seldin 1,4,5,6
1Department of Cell Biology, Emory University School of Medicine, 2Independent scholar, 3Center for Neurodegenerative Disease, Emory University School of Medicine, 4Department of Dermatology, Emory University School of Medicine, 5Winship Cancer Institute, Emory University School of Medicine, 6Atlanta Veterans Affairs Medical Center

The protocol presents a new tool to simplify intravital imaging using inverted confocal microscopy.

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