Hunan provincial key laboratory of cardiovascular research

3 ARTICLES PUBLISHED IN JoVE

Biochemistry

Enhancing the Engraftment of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes via a Transient Inhibition of Rho Kinase Activity

Meng Zhao¹, Yawen Tang¹, Patrick J. Ernst^1,2, Asher Kahn-Krell¹, Chengming Fan^1,3, Danielle Pretorius¹, Hanxi Zhu¹, Xi Lou¹, Lufang Zhou², Jianyi Zhang¹, Wuqiang Zhu¹

¹Department of Biomedical Engineering, School of Medicine, School of Engineering, University of Alabama at Birmingham, ²Division of Cardiovascular Diseases, School of Medicine, School of Engineering, University of Alabama at Birmingham, ³Department of Cardiac Surgery, The Second Xiangya Hospital of Central South University

In this protocol, we demonstrate and elaborate on how to use human induced pluripotent stem cells for cardiomyocyte differentiation and purification, and further, on how to improve its transplantation efficiency with Rho-associated protein kinase inhibitor pretreatment in a mouse myocardial infarction model.

Medicine

Cox-Maze IV Procedure Concomitant with Valvular Surgery In Situs Inversus Dextrocardia: A Single-Center Experience in China

Cheng Luo^*1, Chengming Fan^*1, Hao Zhang¹, Long Song¹, Yuhong Liu¹, Liming Liu¹

¹Department of Cardiovascular Surgery, the Second Xiangya Hospital, Central South University

We summarize the Cox-Maze IV procedure concomitant with valvular surgery performed in patients with situs inversus dextrocardia at this institution.

Medicine

Ultrasound-Guided Induced Pluripotent Stem Cell-Derived Cardiomyocyte Implantation in Myocardial Infarcted Mice

Xun Wu¹, Kele Qin¹, Di Wang², Kun Xiang¹, Jun Peng², Jianjun Guo³, Jinfu Yang^1,2, Chengming Fan^1,2,3

¹Department of Cardiovascular Surgery, Second Xiangya Hospital, Central South University, ²Hunan Provincial Key Laboratory of Cardiovascular Research, ³Hunan Fangsheng Pharmaceutical Co., Ltd.

Ultrasound-guided cell delivery around the site of myocardial infarction in mice is a safe, effective, and convenient way of cell transplantation.