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McGill University Faculty of Medicine and Health Sciences

1 ARTICLES PUBLISHED IN JoVE

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Medicine

Corneal and Limbal Alkali Injury Induction Using a Punch-Trephine Technique in a Mouse Model
Athar Shadmani 1, Hala Shakib Dhowre 1, Ozlem Ercal 1, Xiang Qi Meng 2, Albert Y. Wu 1
1Department of Ophthalmology, Stanford University School of Medicine, 2McGill University Faculty of Medicine and Health Sciences

This protocol describes a method to induce an accurate and reproducible corneal and limbal alkali injury in a mouse model. The protocol is advantageous as it allows for an evenly distributed injury to the highly curved mouse cornea and limbus.

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