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Universidad Europea de Madrid

2 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus
Fabio Cafini *1,2, Nguyen Thi Le Thuy *3, Federico Román 4, José Prieto 5, Sarah Dubrac 6,7, Tarek Msadek 6,7, Kazuya Morikawa 1
1Division of Biomedical Science, Faculty of Medicine, University of Tsukuba, 2Department of Basic Biomedical Science, Universidad Europea de Madrid, 3Human Biology Program, School of Integrative and Global Majors, University of Tsukuba, 4Laboratory of Nosocomial Infections, Department of Bacteriology, Centro Nacional de MicrobiologÍa, Instituto de Salud Carlos III, 5Division of Microbiology, Department of Medicine, School of Medicine, Universidad Complutense, 6Biology of Gram-Positive Pathogens, Department of Microbiology, Institut Pasteur, Paris, France, 7ERL3526, CNRS, Paris, France

We describe here three different protocols for the in vitro investigation of conjugation, transduction, and natural transformation in Staphylococcus aureus.

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Developmental Biology

Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos
María José Blanco *1, Ana I.R. Learte *1, Miguel A. Marchena 1, Emma Muñoz-Sáez 1, María Antonia Cid 1, Iván Rodríguez-Martín 1, Cristina Sánchez-Camacho 2,3
1Faculty of Biomedical and Health Sciences, Universidad Europea de Madrid, 2Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), 3School of Doctoral Studies and Research, Universidad Europea de Madrid

Here we describe the standard protocol for the detection of β-galactosidase activity in early whole mouse embryos and the method for paraffin sectioning and counterstaining. This is an easy and quick procedure to monitor gene expression during development that can also be applied to tissue sections, organs or cultured cells.

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