University of Southern California, Keck School of Medicine

2 ARTICLES PUBLISHED IN JoVE

Biology

Aaron Kolski-Andreaco¹, Haijiang Cai^2,3, D. Spencer Currle⁴, K. George Chandy¹, Robert H. Chow^2,3

¹Department of Physiology and Biophysics, University of California, Irvine (UCI), ²Department of Physiology and Biophysics, University of Southern California, Keck School of Medicine, ³Zilkha Neurogenetic Institute, University of Southern California, Keck School of Medicine, ⁴Department of Developmental and Cell Biology, University of California, Irvine (UCI)

Adrenal medullary chromaffin cell culture systems are extremely useful for the study of excitation-secretion coupling in an in vitro setting. This protocol illustrates the method used to dissect the adrenals and then isolate the medullary region by stripping away the adrenal cortex. The digestion of the medulla into single chromaffin cells is then demonstrated.

Medicine

Point of Care Transcranial Color-Coded Duplex Ultrasound of the Middle Cerebral Artery

Fawaz Philip Tarzi¹, Sebina Bulic¹, Stacey Ho¹, Pravesh Saini¹, Andrei Alexandrov², Yuriy S. Bronshteyn³

¹Department of Neurology, University of Southern California, Keck school of Medicine, ²Department of Neurology, University of Arizona, College of Medicine, ³Department of Anesthesiology, Duke University School of Medicine

Transcranial ultrasound is an essential tool for monitoring patients with various neurological conditions. Although it is commonly used in a protocolized fashion in consultative studies, the brain has been overlooked in many protocols utilizing point-of-care ultrasound (PoCUS). This study proposes a PoCUS image acquisition protocol.