University of California Santa Cruz - UCSC

3 ARTICLES PUBLISHED IN JoVE

Biology

Bacterial Gene Expression Analysis Using Microarrays

Sinem Beyhan¹, Fitnat Yildiz¹

¹Department of Environmental Toxicology, University of California Santa Cruz - UCSC

Bacterial Gene Expression Analysis Using Microarrays

Biology

Vibrio cholerae: Model Organism to Study Bacterial Pathogenesis - Interview

Fitnat Yildiz¹

¹Department of Environmental Toxicology, University of California Santa Cruz - UCSC

Vibrio cholerae: Model Organism to Study Bacterial Pathogenesis - Interview

Biology

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

Chris Troll¹, David Alexander¹, Jennifer Allen¹, Jacob Marquette¹, Manel Camps¹

¹Department of Microbiology & Environmental Toxicology, University of California Santa Cruz - UCSC

Here we demonstrate a simple protocol to create a random mutant library for a given target sequence. We show how this method, which is performed in vivo in Escherichia coli, can be coupled with functional selections to evolve new enzymatic activities.