Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency

3 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Identification and Analysis of Myogenic Progenitors In Vivo During Acute Skeletal Muscle Injury by High-Dimensional Single-Cell Mass Cytometry

Richard Medeiros^1,2

¹Department of Biomedicine, Aarhus University, ²Aarhus Institute of Advanced Studies, Aarhus University

The protocol presented here enables the identification and high-dimensional analysis of muscle stem and progenitor cells by single-cell mass cytometry and their purification by FACS for in-depth studies of their function. This approach can be applied to study regeneration dynamics in disease models and test the efficacy of pharmacological interventions.

Bioengineering

Microtubule Plus-End Dynamics Visualization in Huntington's Disease Model based on Human Primary Skin Fibroblasts

Aleksandra Taran^*1,2, Lilia Belikova (Shuvalova)^*2,3, Svetlana Lavrushkina^1,2, Alexandra Bogomazova^3,4, Maria Lagarkova^3,4, Irina Alieva^1,3

¹A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, ²Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, ³Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, ⁴Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency

This protocol is dedicated to the microtubule plus-end visualization by EB3 protein transfection to study their dynamic properties in primary cell culture. The protocol was implemented on human primary skin fibroblasts obtained from Huntington's disease patients.

Developmental Biology

Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors

Artem Eremeev^1,3, Lilia Belikova^1,2, Evgeny Ruchko¹, Egor Volovikov^1,5, Olga Zubkova¹, Alexy Emelin⁴, Roman Deev⁴, Olga Lebedeva^1,3, Alexandra Bogomazova^1,3, Maria Lagarkova^1,3

¹Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, ²Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, ³Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, ⁴Department of Pathological Anatomy, North-Western State Medical University, ⁵Department of Biology, Lomonosov State University

Here we describe a protocol for generating brain organoids from human induced pluripotent stem cells (iPSCs). To obtain brain organoids in large quantities and of high quality, we use home-made mini bioreactors.