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Bonn Medical School, Institute of Pathology

2 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Production of Chick Embryo Extract for the Cultivation of Murine Neural Crest Stem Cells
Kristian Pajtler *1, Anna Bohrer *1, Jochen Maurer 2, Hubert Schorle 2, Alexander Schramm 1, Angelika Eggert 1, Johannes Hubertus Schulte 1
1Department of Pediatric Oncology, University Children's Hospital Essen, 2Department of Developmental Pathology, Bonn Medical School, Institute of Pathology

To cultivate neural crest stem cells (NCSC) in vitro, a special medium (NCSCM) is required. Essential part of NCSCM is chick embryo extract (CEE). We here describe accurate techniques to produce a maximized amount of pure and high quality CEE, including details as the isolation, maceration, centrifugation, and filtration processes.

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Developmental Biology

Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells
Caroline Kubaczka 1, Hubert Schorle 1
1Department of Developmental Pathology, Institute of Pathology, University of Bonn Medical School

Here we present a protocol for the direct conversion of murine embryonic fibroblasts into fully functional and stable trophoblast stem cells by ten day over-expression of Tfap2c, Gata3, Eomes and Ets2.

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