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The Research Institute at Nationwide Children's Hospital

8 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

In vitro Biofilm Formation in an 8-well Chamber Slide
Joseph A. Jurcisek 1, Amanda C. Dickson 1, Molly E. Bruggeman 1, Lauren O. Bakaletz 1
1Center for Microbial Pathogenesis, The Research Institute at Nationwide Children's Hospital

This article describes the procedure for the formation and visualization of a bacterial biofilm grown within an 8-well chamber slide

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Medicine

Collection and Extraction of Saliva DNA for Next Generation Sequencing
Michael R. Goode 1, Soo Yeon Cheong 1, Ning Li 1, William C. Ray 1,2, Christopher W. Bartlett 1,3
1Battelle Center for Mathematical Medicine, The Research Institute at Nationwide Children's Hospital, 2The Interdisciplinary Graduate Program in Biophysics, The Ohio State University, 3Department of Pediatrics, The Ohio State University

DNA extraction from saliva can provide a readily available source of high molecular weight DNA, with little to no degradation/fragmentation. This protocol provides optimized parameters for saliva collection/storage and DNA extraction to be of sufficient quality and quantity for downstream DNA assays with high quality requirements.

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Biology

Isolation of Murine Valve Endothelial Cells
Lindsey J. Miller 1,2, Joy Lincoln 2,3
1Molecular, Cellular and Developmental Biology Graduate Program, The Ohio State University, 2Center for Cardiovascular and Pulmonary Research, The Heart Center, The Research Institute at Nationwide Children's Hospital, 3Department of Pediatrics, The Ohio State University

The ability to isolate heart valve endothelial cells (VECs) is critical for understanding mechanisms of valve development, maintenance, and disease. Here we describe the isolation of VECs from embryonic and adult Tie2-GFP mice using FACS that will allow for studies determining the contribution of VECs in developmental and disease processes.

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Chemistry

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues
R. Wolfgang Rumpf 1, William C. Ray 1
1Battelle Center for Mathematical Medicine, The Research Institute at Nationwide Children's Hospital

Synthetic protein sequences based on consensus motifs typically ignore co-evolving residues, that imply interpositional dependencies (IPDs). IPDs can be essential to activity, and designs that disregard them may result in suboptimal results. This protocol uses StickWRLD to identify IPDs and help inform rational protein design, resulting in more efficient results.

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Biology

Isolation of Murine Coronary Vascular Smooth Muscle Cells
Kathryn E. Husarek 1,2, Xiaojin Zhang 2, Patricia E. McCallinhart 2, Pamela A. Lucchesi 2,3, Aaron J. Trask 2,3
1School of Biomedical Science, The Ohio State University College of Medicine, 2Center for Cardiovascular and Pulmonary Research, The Research Institute at Nationwide Children's Hospital, 3Department of Pediatrics, The Ohio State University College of Medicine

The purpose of this protocol is to demonstrate the isolation and culture techniques of murine primary vascular smooth muscle cells (VSMCs) from the coronary circulation. Once VSMCs have been isolated, they can be used for many standard culture techniques.

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Engineering

An Available Technique for Preparation of New Cast MnCuNiFeZnAl Alloy with Superior Damping Capacity and High Service Temperature
Dong Li 1,3, Wenbo Liu 1,2, Ning Li 1, Zhenyu Zhong 1, Jiazhen Yan 1, Sanqiang Shi 2
1School of Manufacturing Science and Engineering, Sichuan University, 2Department of Mechanical Engineering, Hong Kong Polytechnic University, 3Department of Mechanical and Electrical Engineering, Chengdu Aeronautic Polytechnic

Here we present a protocol to obtain a novel Mn-Cu-based alloy with excellent comprehensive performances by a high-quality smelting technology and reasonable heat treatment methods.

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Biology

A Labor-saving and Repeatable Touch-force Signaling Mutant Screen Protocol for the Study of Thigmomorphogenesis of a Model Plant Arabidopsis thaliana
Kai Wang 1, Kayin Law 1, Manhin Leung 1, Waishing Wong 1, Ning Li 1,2
1Division of Life Science, Energy Institute, Institute for the Environment, The Hong Kong University of Science and Technology, 2Shenzhen Research Institute, The Hong Kong University of Science and Technology

A gentle touch-force loading machine is built from human hair brushes, robotic arms and a controller. The hair brushes are driven by robotic arms installed on the machine and move periodically to apply touch-force on plants. The strength of machine-driven hair touches is comparable to that of manually applied touches.

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JoVE Core

Home-Based Prescribed Pulmonary Exercise in Patients with Stable Chronic Obstructive Pulmonary Disease
Xiaodan Liu 1,2, Peijun Li 3, Jian Li 3, Lu Xiao 1, Ning Li 3, Yufan Lu 3, Zhengrong Wang 3, Jianqing Su 3, Zhenwei Wang 4, Chunlei Shan 1,2, Weibing Wu 3
1School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, 2Institute of Rehabilitation Medicine, Shanghai Academy of Traditional Chinese Medicine, 3Department of Sports Medicine, Shanghai University of Sport, 4Department of Respiratory Medicine, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine

Presented here is a protocol to investigate the effects of home-based prescribed pulmonary exercise in stable chronic obstructive pulmonary disease (COPD) patients, which is modified based on traditional Chinese exercises according to dyspnea and limited exercise capacity observed in COPD patients.

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