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Korea University College of Medicine

4 ARTICLES PUBLISHED IN JoVE

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Medicine

Use of a Hanging-weight System for Isolated Renal Artery Occlusion
Almut Grenz 1, Julee H. Hong 1, Alexander Badulak 1, Douglas Ridyard 1, Timothy Luebbert 2, Jae-Hwan Kim 3, Holger K. Eltzschig 1
1Department of Anesthesiology, University of Colorado, 2School of Medicine, University of Colorado, 3Department of Anesthesiology, Korea University College of Medicine

A precise murine model for acute kidney injury (AKI) due to ischemia is an important tool to investigate acute kidney injury and possibly find therapeutic tools to treat renal injury. The hanging weight system offers a tool for immediate and reliable renal artery occlusion and reperfusion without causing renal congestion.

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Neuroscience

Heat-Induced Antigen Retrieval: An Effective Method to Detect and Identify Progenitor Cell Types during Adult Hippocampal Neurogenesis
Syed M.Q. Hussaini *1, Heechul Jun *1, Chang Hoon Cho 1, Hyo Jin Kim 1, Woon Ryoung Kim 2, Mi-Hyeon Jang 1,3
1Department of Neurologic Surgery, Mayo Clinic College of Medicine, 2Department of Anatomy, Korea University College of Medicine, 3Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine

During adult hippocampal neurogenesis, a distinct set of genetic markers are expressed when a quiescent neural stem cell sequentially progresses and develops into a functionally integrated neuron in the circuit. Using heat-induced antigen retrieval, progenitor cell types that are otherwise difficult to detect are identified with improved effectiveness.

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Biology

ACT-PRESTO: Biological Tissue Clearing and Immunolabeling Methods for Volume Imaging
Eunsoo Lee 1, Woong Sun 1
1Department of Anatomy, Korea University College of Medicine

ACT-PRESTO (active clarity technique–pressure related efficient and stable transfer of macromolecules into organs) enables rapid, efficient, but inexpensive tissue clearing and rapid antibody penetration into cleared tissues by passive diffusion or pressure-assisted delivery. Using this method, a wide range of tissues can be cleared, immunolabeled by multiple antibodies, and volume-imaged.

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Neuroscience

Isolation and Culture of Adult Neural Stem Cells from the Mouse Subcallosal Zone
Joo Yeon Kim 1, Ju-Hyun Lee 1, Woong Sun 1
1Department of Anatomy, Korea University College of Medicine

Establishing culture systems for the expansion of adult neural stem cells (aNSCs) allows for the examination and application of aNSCs for therapy. The subcallosal zone (SCZ) has recently been recognized as a novel neuroblast-forming region in adult mice. Here, methods for the isolation, expansion, and differentiation of SCZ-aNSCs are described.

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