University Hospital of Würzburg

7 ARTICLES PUBLISHED IN JoVE

Neuroscience

Microelectrode Guided Implantation of Electrodes into the Subthalamic Nucleus of Rats for Long-term Deep Brain Stimulation

Felix Fluri¹, Micheal Bieber¹, Jens Volkmann¹, Christoph Kleinschnitz¹

¹Department of Neurology, University Hospital Wuerzburg

A method for implanting electrodes into the subthalamic nucleus (STN) of rats is described. Better localization of the STN was achieved by using a microrecording system. Furthermore, a stimulation set-up is presented that is characterized by long-lasting connections between the head of the animal and the stimulator.

Behavior

A Novel Approach to Assess Motor Outcome of Deep Brain Stimulation Effects in the Hemiparkinsonian Rat: Staircase and Cylinder Test

Marta Rattka¹, Felix Fluri¹, Miloš Krstić¹, Esther Asan², Jens Volkmann¹

¹Department of Neurology, University Hospital Wuerzburg, ²Institute of Anatomy and Cell Biology, University Wuerzburg

Deep brain stimulation (DBS) is an effective treatment option for Parkinson's disease. We established a study design to screen novel stimulation paradigms in rats. The protocol describes the use of the staircase test and cylinder test for motor outcome assessment in DBS treated hemiparkinsonian rats.

Neuroscience

Stereological Estimation of Dopaminergic Neuron Number in the Mouse Substantia Nigra Using the Optical Fractionator and Standard Microscopy Equipment

Chi Wang Ip¹, David Cheong¹, Jens Volkmann¹

¹Department of Neurology, University Hospital of Würzburg

This work presents a step-by-step protocol for the unbiased stereological estimation of dopaminergic neuronal cell numbers in the mouse substantia nigra using standard microscopy equipment (i.e., a light microscope, a motorized object table (x, y, z plane), and public domain software for digital image analysis.

Neuroscience

Implantation of Osmotic Pumps and Induction of Stress to Establish a Symptomatic, Pharmacological Mouse Model for DYT/PARK-ATP1A3 Dystonia

Lisa Rauschenberger¹, Susanne Knorr¹, Jens Volkmann¹, Chi Wang Ip¹

¹Department of Neurology, University Hospital of Würzburg

We provide a protocol to generate a pharmacological DYT/PARK-ATP1A3 dystonia mouse model via implantation of cannulas into basal ganglia and cerebellum connected to osmotic pumps. We describe the induction of dystonia-like movements via application of a motor challenge and the characterization of the phenotype via behavioral scoring systems.

Neuroscience

Multifactorial Assessment of Motor Behavior in Rats after Unilateral Sciatic Nerve Crush Injury

Susanne Knorr¹, Lisa Rauschenberger¹, Tami Lang¹, Jens Volkmann¹, Chi Wang Ip¹

¹Department of Neurology, University Hospital of Würzburg

We provide a protocol for the assessment of motor behavior via a behavioral test battery in rats after sciatic nerve crush injury.

Medicine

Dynamic Navigation in Endodontics: Guided Access Cavity Preparation by Means of a Miniaturized Navigation System

Wadim Leontiev¹, Thomas Connert¹, Roland Weiger¹, Gabriel Krastl², Eva Magni¹

¹Department of Periodontology, Endodontology and Cariology, University Center for Dental Medicine Basel UZB, University of Basel, ²Department of Conservative Dentistry and Periodontology, University Hospital of Würzburg

Dynamic navigation systems (DNS) provide real-time visualization and guidance to the operator during endodontic access cavities preparation. The planning of the procedure requires three-dimensional imaging utilizing cone beam computed tomography and surface scans. After the export of the planning data to the DNS, access cavities can be prepared with minimal invasion.

Medicine

Guided Endodontics: Three-Dimensional Planning and Template-Aided Preparation of Endodontic Access Cavities

Wadim Leontiev¹, Thomas Connert¹, Roland Weiger¹, Dorothea Dagassan-Berndt², Gabriel Krastl³, Eva Magni¹

¹Department of Periodontology, Endodontology and Cariology, University Center for Dental Medicine Basel UZB, University of Basel, ²Center for Dental Imaging, University Center for Dental Medicine, University of Basel, ³Department of Conservative Dentistry and Periodontology, University Hospital of Würzburg

Guided endodontics describes a template-aided approach for access cavity preparation. The procedure requires cone-beam computed tomography and a surface scan to produce a template. An incorporated sleeve guides the drill to the target point. This allows the preparation of minimally invasive endodontic access cavities in calcified teeth.