An Optic Nerve Crush Injury Murine Model to Study Retinal Ganglion Cell SurvivalZhongshu Tang 1, Shuihua Zhang 1,2, Chunsik Lee 1, Anil Kumar 1, Pachiappan Arjunan 1, Yang Li 1, Fan Zhang 1, Xuri Li 1
1National Eye Institute, NIH, 2Ophthalmology Department, The Second Hospital of Harbin Medical University
This protocol shows how to retrogradely label retinal ganglion cells, and how to subsequently make an optic nerve crush injury in order to analyze retinal ganglion cell survival and apoptosis. It is an experimental disease model for different types of optic neuropathy, including glaucoma.